Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein (LTP11096)

Product Name
Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein (LTP11096)
Catalog Number
LTP11096
Quantity
100ug
Price
$789 In stock
Background
B7-H3, a member of the B7 family of immunomodulatory molecules, is overexpressed in a wide range of solid cancers.B7-H3 binds to activated T cells via an as yet unidentified receptor. In assays using sub-optimal amount so anti-CD3 stimulation, 2Ig?B7?H3 enhances T cell proliferation, T cell interferon-gamma (IFN-gamma) production, and cytotoxic T cells induction.
Description
Recombinant Biotinylated Human B7-H3 (4Ig)/B7-H3b Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gly27-Thr461.
Target
B7-H3 (4Ig)/B7-H3b
Species
Human
Gene/Protein ID
Q5ZPR3-1
Exact Sequence
Gly27-Thr461
Molecular Weight
The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Anti-B7-H3 Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein (LTP11096)
  • 5 Units in Stock

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