Biotinylated Human ACE2/ACEH Protein (LTP11044)

Product Name
Biotinylated Human ACE2/ACEH Protein (LTP11044)
Catalog Number
LTP11044
Quantity
100ug
Price
$789 In stock
Background
ACE2 (Angiotensin I Converting Enzyme 2) is a Protein Coding gene. Diseases associated with ACE2 include Severe Acute Respiratory Syndrome and Neurogenic Hypertension.The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7.
Description
Recombinant Biotinylated Human ACE2/ACEH Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Gln18-Ser740.
Target
ACE2/ACEH
Species
Human
Gene/Protein ID
Q9BYF1-1
Exact Sequence
Gln18-Ser740
Molecular Weight
The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized SARS-COV-2 S1, His Tag at 2ug/ml (100ul/Well). Dose response curve for Biotinylated Human ACE2, His Tag with EC50 of 0.24ug/ml determined by ELISA. See testing image for detail.
Tag
C-His-Avi
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Human ACE2/ACEH Protein (LTP11044)
  • 5 Units in Stock
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