Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (LTP11049)

Product Name
Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (LTP11049)
Catalog Number
LTP11049
Quantity
100ug
Price
$789 In stock
Background
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Description
Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from Expi293 with His tag and Flag tag and Avi tag at the N-terminal. It contains Thr141-Leu285.
Target
BAFF/TNFSF13B/CD257 Trimer
Species
Cynomolgus
Gene/Protein ID
A0A2K5V2X4
Exact Sequence
Thr141-Leu285
Molecular Weight
The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on the Tris-Bis PAGE result.
Activity
Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA. See testing image for detail.
Tag
N-His-Avi,N-Flag
Express System
Expi293
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as a protectant before lyophilization.
Stability and Storage

Reconstituted protein stable at -80 C for 12 months, 4 C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Shipping
Shipped at ambient temperature.
Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration of more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Image
Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein (LTP11049)
  • 5 Units in Stock

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