Biotin-Secretin, human

Biotin-Secretin, human is a synthetic research peptide in the secretin family. Secretin is a linear 27-residue peptide hormone and the endogenous ligand of the secretin receptor, a class B GPCR. Residue-by-residue structure-function studies demonstrate that both receptor binding and receptor activation depend on defined positions throughout the peptide. Secretin fragments and substituted analogs are therefore useful for mapping ligand-receptor contacts and class B GPCR activation mechanisms.

• receptor-binding
• competition assays
• functional signaling assays
• enzymatic-processing or stability studies
• antibody controls
• LC-MS/HPLC method development
• comparative structure-activity experiments

For a fragment, residue-substituted analog, stereochemical variant, labeled peptide, or terminally modified form, experimental behavior should be evaluated for the exact construct rather than assumed to match the native parent peptide. Sequence-dependent solubility, aggregation, adsorption, and stability should be considered when selecting reconstitution and assay conditions.

1. Importance of Each Residue within Secretin for Receptor Binding and Biological Activity
2. Ligand binding and activation of the secretin receptor, a prototypic family B GPCR