Scientific Background | Biotin-C-EKFAQTVMT(pS)RIVGTTAYMAPKK is a synthetic MAP kinase substrate/phosphopeptide. MAP kinases are proline-directed Ser/Thr kinases whose peptide substrates commonly contain a Ser/Thr-Pro phosphoacceptor context, while docking motifs can further determine isoform selectivity. Defined MAPK substrate and phosphopeptide reagents are useful for phosphorylation kinetics, antibody validation, docking studies, and comparison of kinase isoforms. |
Experimental Notes | The exact sequence, phosphorylation state, residue numbering, terminal modifications, labels, and species context should be matched to the intended assay. Sequence motifs support experimental interpretation but do not by themselves establish absolute enzyme specificity or native biological activity. |
