Anti-ERK1 (E19) Monoclonal Antibody

Catalog Number:
LT9998
Lot Number:
411005100214
Isotype:
Mouse IgG1
Clonality:
E19
Quantity:
50 μg
Immunogen:
Recombinant full length ERK1 protein
Specificity:
Recognizes only the ERK1 protein (e.g.: from human and mouse cells)
Cross Reactivity:
WB: ERK1 from human and mouse cells. IP: ERK1 from human cells. ERK1 from other species may also be detectable
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
IP, WB
Working Condition:
WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)
For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-ERK1 (E19) mAb dilution used in WB of HEK293 and Jurkat cell lysateanti-ERK1 (E19) mAb dilution used in WB of HEK293 and Jurkat cell lysate
Fig. LEFT: 1:10,000 (0.1µg/mL) Ab dilution used in WB of HEK293 cell lysate; 1µg (1), 5µg (2), and 10µg (3) of cell lysate used; RIGHT: IP of anti-ERK1 using 50µg of Jurkat cell lysate; 0.5µg (1), 1µg (2), and 2µg (3) of anti-ERK1 used
  • 1 Units in Stock

$260.00

Qty Discounts Off Price
1-9
$260.00
10-19
$234.00
20-29
$208.00
30+
$182.00
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