alpha-Helical CRF (12-41)

alpha-Helical CRF (12-41) is a synthetic research peptide in the corticotropin-releasing factor/hormone (CRF/CRH) family. CRF/CRH is a 41-residue peptide hormone that activates CRF receptors and plays a central experimental role in studies of hypothalamic-pituitary-adrenal signaling. Helical propensity and defined receptor-interaction regions have been exploited to create CRF fragments, constrained analogs, and antagonists, which are useful for receptor binding, signaling, and peptide structure-function experiments.

• receptor-binding
• competition assays
• functional signaling assays
• enzymatic-processing or stability studies
• antibody controls
• LC-MS/HPLC method development
• comparative structure-activity experiments

For a fragment, residue-substituted analog, stereochemical variant, labeled peptide, or terminally modified form, experimental behavior should be evaluated for the exact construct rather than assumed to match the native parent peptide. Sequence-dependent solubility, aggregation, adsorption, and stability should be considered when selecting reconstitution and assay conditions.

1. Conformationally restricted competitive antagonists of human/rat corticotropin-releasing factor
2. Potent structurally constrained agonists and antagonists of corticotropin-releasing factor