AKT/PKB/Rac - Protein Kinase Substrate

AKT/PKB/Rac - Protein Kinase Substrate is a synthetic research peptide in the AKT/PKB peptide substrate family. AKT/PKB is a Ser/Thr kinase whose preferred phosphorylation motif has been defined using peptide and protein library screening. Synthetic AKT substrates are used to quantify AKT catalytic activity and test how residues surrounding the phosphoacceptor site contribute to recognition.

• AKT/PKB kinase assays
• substrate-motif studies
• phosphorylation kinetics
• AKT inhibitor screening

The exact sequence, reporter group, phosphorylation state, stereochemistry, and terminal modifications should be considered when comparing this reagent with the parent peptide or published assay substrates. Enzyme concentration, buffer composition, cofactors, pH, ionic strength, and substrate concentration can also affect measured activity.

1. Peptide and protein library screening defines optimal substrate motifs for AKT/PKB