Akt Specific Substrate Peptide, Akt/PKB

Akt Specific Substrate Peptide, Akt/PKB is a synthetic defined protein-kinase substrate or regulatory peptide. Synthetic kinase substrate peptides are used to measure phosphorylation, characterize sequence specificity, compare kinase activity, and develop biochemical assays. Kinase recognition depends on residues surrounding the phosphoacceptor and, for some kinases, additional docking motifs; therefore a peptide described for one kinase should not automatically be considered absolutely kinase-specific.

• in vitro kinase activity assays
• substrate-specificity and phosphorylation studies
• kinase inhibitor screening
• phosphopeptide and analytical assay development

The exact sequence, phosphorylation state, residue numbering, terminal modifications, labels, and species context should be matched to the intended assay. Sequence motifs support experimental interpretation but do not by themselves establish absolute enzyme specificity or native biological activity.

1. Characterization of kinase target phosphorylation consensus motifs using peptide arrays
2. Rapid determination of linear kinase substrate motifs by mass spectrometry
3. Atlas of substrate specificities for the human serine/threonine kinome