Product Name | Ac-IEAR-pNA |
Product Quantity | 10mg |
Catalog Number | LT0848 |
Molecular Weight | 649.7 |
Formula | C28H43N9O9 |
Sequence | Ac-Ile-Glu-Ala-Arg-pNA, Ac-IEAR-pNA |
Product Description | Ac-IEAR-pNA is a peptide substrate used in laboratories to measure the activity of caspases. Ac-IEAR-pNA is also a cholinesterase inhibitor that is used to measure the activity of butyrylcholinesterase. This enzyme hydrolyzes acetylcholine into butyrylcholine and acetic acid. The Ac-IEAR-pNA peptide is the chromogenic substrate for quantifying endotoxin levels. The kinetic chromogenic endotoxin assay uses a bacterial endotoxin to activate a protease in Limulus Amebocyte Lysate (LAL) that hydrolyzes the colorless substrate Ac-Ile-Glu-Ala-Arg-pNA, resulting in the production of p-nitroaniline. The chromogenic substrate Ac-IEAR-pNA can be cleaved by human butyrylcholinesterase (HuBChE) to produce the yellow p-nitroaniline product detected at 415 nm. |
Scientific Background | Ac-IEAR-pNA is a 4-residue synthetic peptide with the sequence Ile-Glu-Ala-Arg. N-terminal acetylation neutralizes the terminal alpha-amino group. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence. |
Research Applications | - LC-MS/HPLC analytical method development
- sequence-specific assay controls
|
Experimental Notes | Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions. |
