Ac-Gly-Ala-Lys(Ac)-AMC

(image for) Ac-Gly-Ala-Lys(Ac)-AMC
Product Name
Ac-Gly-Ala-Lys(Ac)-AMC
Product Quantity
4mg
Catalog Number
LT2537
Molecular Weight
515.57
Formula
C25H33N5O7, CAS Number: 577969-56-3
Sequence
Ac-GAK(Ac)-AMC is a fluorogenic substrate for measuring histone deacetylase class I and class II activity in a protease-coupled assay. HDAC-catalyzed deacetylation of Lys yields Ac-GAK-AM.
Scientific Background

Ac-Gly-Ala-Lys(Ac)-AMC is a 11-residue synthetic peptide with the sequence Asp-Glu-Ala-Cys-Glu-Thr-Tyr-Leu-Ala-Ser-Glu. N-terminal acetylation neutralizes the terminal alpha-amino group. The sequence contains 1 cysteine residue, providing potential thiol/disulfide chemistry when the thiol is available; contains 1 Asp and 3 Glu residues, contributing anionic character near neutral pH. These sequence-derived properties describe the reagent chemically; no specific receptor, enzyme, pathway, disease association, or biological activity is assigned without product-specific experimental evidence.

Research Applications
  • thiol-selective conjugation or immobilization studies
  • LC-MS/HPLC analytical method development
  • sequence-specific assay controls
Experimental Notes

Sequence-derived chemical properties support reagent selection and experimental planning but do not establish biological function. Solubility, aggregation, adsorption, conjugation efficiency, and assay performance should be validated under the intended experimental conditions.

  • 5 Units in Stock
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$530.00

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