Can you split the purified peptide so that half the sample could be labeled with a biotin tag and the remaining is left unlabled. Do I need to quote for two peptides? This is for N terminal labeling.
The label biotin is conjugated with the peptide before cleaving from the resin. After purification, we have no way to conjugate it with the peptide. We need to quote as two different peptides as purification will be performed differently.