Fibrin Hydrogels are a widely used material in regenerative medicine, often made with fibrinogen obtained from human plasma. However, this process can be very costly and have problems when used in other applications due to their limited mechanical properties. Researchers were interested in the prospect of composite hydrogels, made from the self-assembly of fibrinogen together with Fmoc-FF and Fmoc-RGD. Their results saw that these hydrogels co-assembled from short peptides and fibrin display biocompatibility, as well as the enhanced mechanical properties they were looking for.
Fibrin hydrogel enhanced with short peptide co-assembly
LifeTein provided the group with the essential peptides, Fmoc-FF and Fmoc-RGD, where the team tested different ratios of these peptides in combination with the fibrin precursor. Even from the naked eye alone, they could immediately tell the difference when the peptides were added in the presence of CaCl2. The resulting mixture displayed very favorable and biocompatible characteristics, even similar to the fibrin hydrogels it was replicating. The mixture could even jellify in situ, potentially allowing the pregel to be administered via injection
The protocol the group explored holds great potential for the future of regenerative medicine. Not only do they not cause any inflammatory response, but the possibilities still exist to modify these fibrin hydrogels for even further medicinal usage. The Fmoc-FF and Fmoc-RGD peptides used for the co-assembly of this hydrogel are commercially available, and companies like LifeTein are always ready to supply vital peptides like these to any groups researching to better our future.
Cristina Gila-Vilchez, Mari Carmen Mañas-Torres, Óscar Darío García-García, Alfredo Escribano-Huesca, Laura Rodríguez-Arco, Víctor Carriel, Ismael Rodriguez, Miguel Alaminos, Modesto Torcuato Lopez-Lopez, and Luis Álvarez de Cienfuegos ACS Applied Polymer Materials 2023 5 (3), 2154-2165 DOI: 10.1021/acsapm.2c02164
Oncolytic viruses are a novel type of virus that specifically targets and eliminates tumor cells, and have been an emerging immunotherapy agent. One such is Coxsackievirus A21 (CVA21), which has been demonstrating a healthy safety profile as well as desired targeting of the cancerous cells. While producing drugs that can properly utilize this virus, there rises a need to characterize the ratio of total particles to empty ones, thus researchers developed a method to see Coxsackievirus A21 viral proteins quantified with capillary Western and peptides made up of the components of the capsid proteins.
CVA21 characterized with capillary Western and capsid peptides
LifeTein provided the group with the capsid peptides, VP1, VP2, VP3, and VP4, as well as the antibodies necessary for the testing. Results utilizing these peptides and antibodies included total particle concentration, empty particle concentration, as well as the empty to full ratio. Being able to characterize this kind of information in this downstream process will prove to be immensely valuable in future studies.
The Simple Western method displayed by the researchers proved itself to be an invaluable tool for optimizing decisions for these types of oncolytic virus studies. Consistency in these tests will surely lead to massive amounts of time being saved in future drug development and assessments. LifeTein will always be ready to lend a hand to any work needing peptides or antibodies to further their research in the broad field of drug application.
Paul F. Gillespie, Richard R. Rustandi, Andrew R. Swartz, Liang Shang, Jessica Raffaele, Ashley Prout, Nicholas Cunningham, Mohamed Dawod, James Z. Deng, Shiyi Wang, Jessica Olson, Yvonne Shieh, and John W. Loughney. Quantitation of Coxsackievirus A21 Viral Proteins in Mixtures of Empty and Full Capsids Using Capillary Western. Human Gene Therapy.Jan 2023.68-77.http://doi.org/10.1089/hum.2022.147
Citrus greening, or Huanglongbing (HLB), is a disease that devastates citrus production all over the world. The culprit behind HLB is the bacterium Candidatus Liberibacter spp. (e.g., CLas), an unculturable pathogen that has proven very difficult to treat. Once a tree is infected, it becomes unproductive and dies within years, costing the global citrus market billions. While current attempts to combat HLB rely on controlling the insect vector, scientists have turned some attention towards the potential of peptides. Their work displayed how antimicrobial peptides show promise for combatting citrus greening, mainly by methods against CLas itself.
Antimicrobial peptides effective against CLas bacteria
With not many current effective options to fight HLB, scientists believe the next area of area of interest was targeting the CLas secretory pathway using antimicrobial peptides provided by LifeTein. Specifically, the antimicrobial peptides would be blocking the TolC efflux pump protein. The study found three peptides capable of doing this by binding tightly with the TolC receptors, and even the β barrel entrance of the protein as well. Treatment with peptides in this manner showed effective inhibition and even mortality in models closely resembling CLas.
The studies displayed using antimicrobial peptides show major promise for future treatment of HLB. With the chemical resistant bacteria CLas being nearly impossible to slow down, peptides just may have been holding the solution all along. There is hope that new therapies can be developed utilizing the strategies shown, and the global citrus production can rest easy after decades worth of HLB ravaging the farms.
Wang, Haoqi, Nirmitee Mulgaonkar, Samavath Mallawarachchi, Manikandan Ramasamy, Carmen S. Padilla, Sonia Irigoyen, Gitta Coaker, Kranthi K. Mandadi, and Sandun Fernando. 2022. “Evaluation of Candidatus Liberibacter Asiaticus Efflux Pump Inhibition by Antimicrobial Peptides” Molecules 27, no. 24: 8729. https://doi.org/10.3390/molecules27248729
Parkinson’s Disease is an ever-prevalent neurodegenerative disease whose cases are expected to double within the next decade. While it is a multifactorial disorder that may stem from a combination of environmental and genetic factors, more recent study has shifted the spotlight onto gut microbiota and microbial pathogens as a large contributor to neurodegenerative diseases altogether. Specifically, curli-producing bacteria in the gut microbiota could increase and facilitate alpha-synuclein aggregation in the brain. This spear-headed some to research more into the Curli and HSV-1 antibody correlation shown in Parkinson’s Disease patients.
Curli-producing bacteria in gut could lead to neurodegenerative diseases
LifeTein supplied the group pursuing this work with an immunogenic peptide derived from bacterial amyloid curli to help test their theory. They chose to analyze the humoral response against this peptide as well as other α-syn peptides in both Parkinson’s Disease patients and healthy controls. What was found was a significant correlation in the Parkinson’s Disease patients’ humoral response against curli and HSV-1 when compared to the control.
While the study shows this high correlation exists between these bacterial peptides and Parkinson’s Disease, it is only the first step in this journey. The role of each of these pathogens in Parkinson’s Disease is yet to be explored, and still offers a new angle of understanding the neurodegenerative disease, and hopefully a path for treating it as well.
Jasemi S, Paulus K, Noli M, Simula ER, Ruberto S, Sechi LA. Antibodies against HSV-1 and Curli Show the Highest Correlation in Parkinson’s Disease Patients in Comparison to Healthy Controls. International Journal of Molecular Sciences. 2022; 23(23):14816. https://doi.org/10.3390/ijms232314816
Influenza A is a virus responsible for multiple pandemics over the last centuries, the respiratory disease has claimed millions of lives over the course of human history. Though other pandemics may come to mind in recent years, flu season is just starting up again now as the weather gets chillier. While we can fight back the virus on a yearly basis, more understanding is requited for a long-term victory. A team has been researching into the NS1 protein of the virus, the part responsible for downregulating the antiviral response of host cells to facilitate viral replication. They believe their work has revealed information on the vital role of 14-3-3γ in influenza A virus replication, where the isoform was found to interact with the protein.
Truncated N-Terminus interacts with 14-3-3γ
The group preformed much work such through immunoprecipitation to show the interactions between 14-3-3γ and the influenza A encoded NS1 protein. Some of their most compelling finds was the inhibition of 14-3-3γ expression in the host cells greatly reduced replication of the PR8 wild-type virus, but had no such effect on the R8-NS1/1-98 mutant virus, which lacks most of the effector domain of NS1. LifeTein was able to provide the group with anti β-tubulin antibodies, which assisted in their immunoprecipitation methods.
The team insists that the evidence points directly towards the vital role of 14-3-3γ in influenza A virus replication thanks to the NS1 protein. While they are still unclear on the precise mechanisms of these interactions, they are certain the findings have laid out the groundwork for future pivotal studies involving influenza A and the role of 14-3-3γ in infection.
Kuo, R.-L.; Tam, E.-H.; Woung, C.-H.; Hung, C.-M.; Liu, H.-P.; Liu, H.M.; Wu, C.-C. Interactome Profiling of N-Terminus-Truncated NS1 Protein of Influenza A Virus Reveals Role of 14-3-3γ in Virus Replication. Pathogens 2022, 11, 733. https://doi.org/10.3390/pathogens11070733
The concept of self-assembling peptides is a promising front where construction of devices can be achieved through a single molecule. While the outcome is enticing, the means to reach a consistent outcome are complex to say the least. Dozens of factors go into how a peptide may self-assemble and fold, with the most important being the sequence itself. While this can be handled by careful screening and simulations, the interface at which this folding occurs becomes more important to consider at well. Researchers looked to test how specific peptides fold and self-assemble on graphite-water interfaces, where a number of factors give this method the advantage over doing so in free solution.
Graphite helps peptides self fold into conformations
The group studying this phenomenon claimed that the folded conformations of the peptides were stable over a variety of temperatures when observed over graphite. They point out that it is due to the peptide backbone aligning with the zigzag directions of the graphite plane, thus allowing the conformations to occur more favorably from the intermolecular hydrogen bonds of the molecule. Atomic force microscopy revealed these theories to be true beyond initial simulations as well.
The team believes the design principles displayed in these experiments could be of great use in future iterations of self-assembling peptide engineering. The thermodynamically favored self-assembly with the use of a graphite-water interface shows promise as a medium for even more complex molecular devices in the future, a future LifeTein is looking forward to being a part of.
Justin Legleiter, Ravindra Thakkar, Astrid Velásquez-Silva, Ingrid Miranda-Carvajal, Susan Whitaker, John Tomich, and Jeffrey Comer Journal of Chemical Information and Modeling 2022 62 (17), 4066-4082 DOI: 10.1021/acs.jcim.2c00419
The Nobel Prize in Chemistry 2022 awarded to Carolyn R. Bertozzi, Morten Meldal, and K. Barry Sharpless for their outstanding work with click chemistry, a well-deserved honor for developing a vital and straightforward technique in modern chemistry. Barry Sharpless, with this as his second Nobel Prize in Chemistry, and Morten Meldal independently presented the idea of click chemistry over twenty years ago. The reactions involved are highly versatile and can be performed under a multitude of conditions, making the method incredibly efficient and applicable to many fields. In a perfect example, Carolyn Bertozzi, who was jointly awarded this Nobel Prize, utilized the principles of click chemistry to present bioorthogonal chemistry, click reactions that take place within living organisms without disrupting the normal chemistry of the cell. Since its inception, click chemistry has had its influence explored across many other facets of chemistry, including very useful applications in peptide synthesis.
LifeTein frequently uses this CuAAC reaction in its oligonucleotide-peptide conjugation service. Such reactions would not be nearly as feasible without the simple answer of click chemistry, as linking large and functionalized molecules like peptides and oligonucleotides is a very challenging task otherwise. With this, LifeTein is able to provide DNA-peptide conjugates and RNA-peptide conjugates for cell screening and in vivo studies with ease.
Congratulations again to Carolyn R. Bertozzi, Morten Meldal, and K. Barry Sharpless on their joint award for The Nobel Prize in Chemistry 2022, the field wouldn’t be anywhere near where it is today without click chemistry.
Lyme disease is an abundantly present vector-born disease, with nearly 400,000 new cases in the U.S. diagnosed each year. While treatment with antibiotics is effective, if the disease goes unnoticed and unchecked it can lead to lifelong damage to the nervous and musculoskeletal system. Hence, it is critical to detect the disease as early as possible, however this becomes a complex issue since not every case results in erythema migrans (EM), the characteristic skin lesion of Lyme disease. This issue is further complicated since current serodiagnostics lack sensitivity in early Lyme disease detection, when treatment would be the most effective. One group sought to solve this issue by developing a method more sensitive and more specific than the current ones, ultimately using linear peptide epitomes to better diagnose Lyme disease.
Linear peptide epitomes lead to higher specificity and sensitivity in Lyme disease assays
LifeTein synthesized peptides from prominent B. burgdorferi antigens expressed during human infection of Lyme disease for the group to utilize. Their idea is to use synthetic peptides that contain the linear epitomes necessary, rather than protein antigens that often have cross-reactive epitomes. Eliminating the cross-reactive epitomes leads to more specificity within the tests, and more sensitivity as well. While the results for the single peptide epitomes were less than anticipated, the group found that using the epitome peptides in pairs resulted in the specificity and sensitivity they had hypothesized.
Diagnostic assessment of Lyme disease is in dire need of an improvement to efficiently catch it in the early stages, and these linear peptide epitomes are shaping to be a key part of the solution. The group hopes that their research will allow future works to improve upon their studies and one day include the linear peptide epitomes as part of a potential multi-peptide-based assay for the laboratory diagnosis of Lyme disease.
Arnaboldi PM, Katseff AS, Sambir M, Dattwyler RJ. Linear Peptide Epitopes Derived from ErpP, p35, and FlaB in the Serodiagnosis of Lyme Disease. Pathogens. 2022; 11(8):944. https://doi.org/10.3390/pathogens11080944
As the world moves on through the ongoing pandemic that is COVID-19, great minds across every field and corner of the world are doing their part to further our collective understanding of the virus. A recent study specifically took greater notice in the population of antibodies increasing in more sever cases of COVID-19 over mild ones. Specifically, there was a study conducted on the higher HERV-W and IFN-I antibody presence detected in intensive care COVID-19 patients, utilizing peptides for the experiment.
HERV-W and IFN-I antibody presence higher in severe COVID-19 cases
LifeTein supplied the group with the HERV-W-env(248–262), IFN-α, and IFN-ω peptides necessary for the report. Not only did the studies prove higher levels of anti-IFN-I autoantibodies and HERV-W antibodies are much more prevalent in severe COVID-19 cases than in mild ones, but it opened up a new perspective on how the humoral responses against HERV-W-env(248–262) and IFN-α are correlated across ICU patients. Hopefully, this research leads to more beneficial breakthroughs over time, for COVID-19 cases and potentially other life-threatening conditions as well.
Simula, E. R., Manca, M. A., Noli, M., Jasemi, S., Ruberto, S., Uzzau, S., Rubino, S., Manca, P., & Sechi, L. A. (2022). Increased Presence of Antibodies against Type I Interferons and Human Endogenous Retrovirus W in Intensive Care Unit COVID-19 Patients. In H. H. Mostafa (Ed.), Microbiology Spectrum. American Society for Microbiology. https://doi.org/10.1128/spectrum.01280-22
Analyzing cell interactions was always vital for biological studies, but a simple approach from microscopy fails to provide any information on receptors and ligands involved in these interactions. Scientists have developed a method of bacterial sortase labeling involving a fluorescent LPXTG peptide motif and Staphylococcus aureus transpeptidase Sortase A (SrtA) that can be readily detected using flow cytometry. The group coined this approach as Labeling Immune Partnerships by SorTagging Intercellular Contacts, or LIPSTIC for short. LIPSTIC is even more useful in the fact that the LPETG peptide and SrtA reaction allows analysis of cell-cell interactions both in vitro and in vivo.
LPETG peptide and SrtA reaction to label receptor and ligand interactions.
LifeTein supplied the group with the necessary Biotin-ahx-LPETG peptide, where in the LIPSTIC method a ligand or receptor of note that is fused with a tag consisting of five N-terminal glycine residues (G5) has the fluorescent peptide donated to it by the SrtA. The acceptor cell can then be monitored via the label after separation. The group is confident that LIPSTIC is an efficient method to label receptor-ligand interactions both in vitro and in vivo, even able to detect rare or low-intensity interactions.
Pasqual G, Chudnovskiy A, Tas JMJ, Agudelo M, Schweitzer LD, Cui A, Hacohen N, Victora GD. Monitoring T cell-dendritic cell interactions in vivo by intercellular enzymatic labelling. Nature. 2018 Jan 25;553(7689):496-500. doi: 10.1038/nature25442. Epub 2018 Jan 17. PMID: 29342141; PMCID: PMC5853129.