Product Name:Recombinant Mouse RANKL (E. coli-expressed, Active)

Related Product: m-CSF Protein (Cat. No. LT13501)

Catalog Number: LTP4418

Product Size: 25µg

Transportation method:Shipped at Room temp

Uniprot ACC#: O35235

Subcategory:RANK Ligand

Amino acid sequence: E. coli-derived mouse RANKL protein

Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 50ng/mL.

Formulation:The sRANKL Mouse was lyophilized from a 0.2µgm filtered concentrated solution in PBS with 0.1% FBS.

Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.

Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Source:Escherichia Coli.

Stability:Lyophilized RANKL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution sRANKL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Synonyms: Osteoclast differentiation factor (ODF), Osteoprotegerin ligand (OPGL), Receptor activator of NF-?B Ligand (RANKL), TNF-related activation?induced cytokines (TRANCE)

Usage:LifeTein's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

A. The size-exclusion chromatography of recombinant mouse RANKL and the SDS-PAGE analysis. B. The bone marrow-derived monocytes were cultured in the presence of M-CSF(25 ng/ml, (Cat. No. LT13501)) and RANKL (50 ng/ml) for 6 days. The cells were then fixed with 4% PFA and stained by the TRAP solution. The image showed the formation of big multiple nucleus TRAP-positive osteoclasts formed after M-CSF and RANKL stimulation.