Product Name:Pyruvate Kinase I E.Coli Recombinant
Catalog Number: LTP3647
Product Size: 20µg
Transportation method:Shipped with Ice Packs
Uniprot ACC#: P0AD61Enzymes
Subcategory:Protein Kinases
Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LKAHGGENIH IISKIENQEG LNNFDEILEA SDGIMVARGD LGVEIPVEEV IFAQKMMIEK CIRARKVVIT ATQMLDSMIK NPRPTRAEAG DVANAILDGT DAVMLSGESA KGKYPLEAVS IMATICERTD RVMNSRLEFN NDNRKLRITE AVCRGAVETA EKLDAPLIVV ATQGGKSARA VRKYFPDATI LALTTNEKTA HQLVLSKGVV PQLVKEITST DDFYRLGKEL ALQSGLAHKG DVVVMVSGAL VPSGTTNTAS VHVL.
pykF E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 494 amino acids (1-470 a.a) and having a molecular mass of 53.3kDa.pykF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Formulation:pykF protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.
Physical Appearance:Sterile Filtered colorless solution.
Purity:Greater than 95% as determined by SDS-PAGE.
Source:E.coli.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Synonyms: Pyruvate kinase I, PK-1, pykF, b1676, JW1666.
Usage:LifeTein's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
