Product Name:Leptin Antagonist Triple Mutant Ovine Recombinant, Pegylated
Catalog Number: LTP4328
Product Size: 20µg
Transportation method:Shipped at Room temp
Uniprot ACC#: Growth Factors
Subcategory:Leptin
Amino acid sequence:
Pegylated Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 35.6 kDa, Leptin was mutated, resulting in L39A/D40A/F41A mutant. However due to enlarged hydrodymanic volume it runs on the SGS-Page as 48 kDa protein and in gel-filtration on Superdex 200 as over 200kDa protein. Leptin Antagonist Triple Mutant Ovine Recombinant Mono-Pegylated with 20kDa PEG and was purified by proprietary chromatographic techniques.
Formulation:The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
Physical Appearance:White lyophilized (freeze-dried) powder.
Purity:Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
Source:Escherichia coli.
Stability:Lyophilized Leptin Antagonist Triple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18_C. Upon reconstitution at > 0.1 Lep-tA mutant mg/ml and up to 2mM and filter sterilization Leptin mutant can be stored at 4_C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles.
Synonyms:
Usage:LifeTein's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.