Product Name:Fibroblast Growth Factor-basic Human Recombinant, Thermostable
Catalog Number: LTP4204
Product Size: 10µg
Transportation method:Shipped at Room temp
Uniprot ACC#: P09038Growth Factors
Subcategory:Fibroblast Growth Factor
Amino acid sequence:
FGF-Basic Thermostable is a stabilized form of FGF-2 growth factor which enables a novel method to produce FGF2-dependent cell cultures more efficiently, having less media changes. FGF-Basic Thermostable can maintain its biological activity even after five days at 37°C. The increase in the stability of FGF2 in cell-culture enables a more homogenous, undifferentiated stem cell culture, while saving scientists crucial time and money, as frequent supplementation of FGF-basic and the everyday medium change is not necessary. Thermostable FGF-2 is a hyperstable protein. The Thermal stability of the protein is increased by 15°C compared to the wild-type FGF-2. Thermostable FGF-2 is more than 5-times prolonged half-life in human cell culture incubated at 37°C. The FGF-2 Thermostable protein is engineered with fully retained biological function and has no harmful stabilizing additives.Thermostable FGF2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids including a 20 aa His tag and thrombin cleavage recognition site at N-terminus. The total calculated molecular mass is 19.4kDa.
Formulation:FGF2 was filtered and lyophilized from a solution containing 20mM potassium phosphate buffer and 750mM sodium chloride, pH 7.5.
Physical Appearance:Filtered White lyophilized (freeze-dried) powder.
Purity:Purity is greater than 95% as determined by SDS-PAGE.
Source:Escherichia Coli.
Stability:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Synonyms: Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.
Usage:LifeTein's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
