Anti-human Myoglobin Monoclonal Antibody (clone 7D3)

$250.00

Catalog Number:
LT_R012
Lot Number:
 
Immunogen:
Full length recombinant human Mb protein
IgG type:
Rabbit IgG
Clonality:
Monoclonal
Applications:
ELISA, ITA
Pairing antibody:
LT_R011, LT_R013, LT_R014
Specificity:
Human Mb
Formulation:
0.22 μM filtered solution of PBS, pH 7.4
Purity:
> 95% determined by SDS-PAGE
Storage:

This antibody is shipped at 4 °C. This product is stable for 12 months from date of receipt when stored at -20 °C to -70 °C. Avoid freeze/thaw cycles.

Preparation:

Monoclonal antibody is produced by immunizing rabbit with full length human Mb and purified using protein A resin.

Hazard/Biohazard:

This antibody contains 0.09% sodium azide as preservative. Please handle and dispose the product properly. No known biohazard is associated with this product.

Background:

Myoglobin (Mb) is an iron- and oxygen-binding protein which
is expressed in vertebrate skeletal and cardiac muscles. It
contains one polypeptide chain and one heme group. It plays
an essential role in the storage and transport of oxygen to
mitochondria. Reversible oxygen binding occurs by a linkage
with the imidazole nitrogen of the 91st histidine residue in
the myoglobin chain. Myoglobin is released rom damaged
muscle tissue, making it a sensitive marker for muscleinjury
and an important clinical marker for heart attack in patients
with chest pain.

Images:


Fig. 1 ELISA

ELISA conditions
1) capture antibody: rabbit anti- Mb monoclonal antibody (clone 2F11, LT_R014) at 1 μg/ml
2) detection antibody: rabbit anti-Mb monoclonal antibody (clone 7D3, LT_R012) at 0.5 μg/ml


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  • 4 Units in Stock

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