D-amino acid peptides to resist common proteases

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Many proteins are easily degraded or tagged for selective destruction in cells. Peptides that are at least partially made of D-amino acids have shown strong resistance to proteolytic degradation.

D amino acid peptide with high stability

D amino acid peptide with high stability

D-amino acids have been detected in a variety of peptides synthesized by animal cells. These include opiate and antimicrobial peptides from amphibian skin, neuropeptides from snail ganglia, hormones from crustaceans, and venom compounds from spiders. These D-amino acids form when L-amino acids undergo a posttranslational reaction. One prototype enzyme catalyzing this type of reaction has recently been isolated from spider venom.

In omega-agatoxins IVB and IVC peptides with D-serine at position 46 are about four times more potent than those with L-serine isomer with respect to inhibitory action on P-type Ca channels in rat cerebellar Purkinje cells.

The peptide bonds formed by D-amino acids are more resistant to proteases than those of L-amino acids. For example, the amino-terminal Tyr-D-Ala sequence of dermorphin is not hydrolyzed by aminopeptidases, but the corresponding L-peptide is rapidly degraded.

Peptides can be modified to be stable against proteolysis while still displaying the same binding properties as their original all-L counterparts. Studies have been performed on the antigenic properties and enzymatic stability of several MUC2 peptides whose residues were replaced with D-amino acids in the flanking regions.

Studies have shown that the peptide tp-TPTGTQ-tpt with D-amino acids in the flanks retained full antibody binding properties and demonstrated a pronounced resistance to proteolytic degradation in diluted human serum and lysosomal preparation. (Here, lowercase letters denote D amino acids and capital letters denote L-amino acids)

In a study by Tugyi et al, D-amino acids present at the N termini of various peptides (tPTPTGTQTPT and tptPTGTQTPT) provided improved, but not complete, stability in both 10% and 50% human serum. However, substitutions at both ends of the peptide produced compounds that were almost immune to serum degradation.

For example, the peptide tPTPTGTQtpt was more stable than TPTPTGTQtpt, and tpTPTGTQtpt was completely stable. Note that the stability of each peptide increased with the number of D-amino acids at the C terminus: One D-amino acid at the C terminus (tptPTGTQTPt) improved stability, though the peptide was still degradable. Two D-amino acids at the C terminus (tptPTGTQTpt) produced a peptide that remained completely stable in 50% human serum throughout the assay.

These findings also indicate that, combinations of structural modifications (D-amino acid substitution) in the flanks of antibody epitope may be used to construct a synthetic antigen that has both the recognition properties of the original antibody and strong resistance to enzymatic degradation.

Reference: http://www.pnas.org/content/102/2/413.full.pdf+html

Amino acid composition of cell-penetrating peptides (CPPs)

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Cell-penetrating peptides (CPPs) such as the HIV TAT peptides are able to enter cell by direct translocation and endocytosis. HIV TAT or even simple poly-arginines can be effectively designed for drug delivery. However how cell-penetrating peptides, HIV TAT peptide for example, accomplish these cellular molecular transfer has so far been a mystery.

How does simple HIV TAT peptide facilitate mechanisms like direct translocation and multiple endocytotic processes? Researchers from Gerard Wong’s lab found how HIV TAT peptides can have multiple interactions with the cell membrane, the actin cytoskeleton and specific cell-surface receptors to produce multiple pathways of translocation under different conditions. Click here for the publication from Gerard Wong’s lab: http://bit.ly/zQrH6t.

cell penetrating peptide entry mechanism

Cell Penetrating Peptides

Interestingly, TAT peptide can multiplex different interactions with the same sequence, thus interacting with the membrane, the actin cytoskeleton, and specific receptors to produce multiple pathways of translocation under different conditions.

CPPs entry mechanism is sensitive to the peptide sequence. The addition of a single hydrophobic residue to purely hydrophilic CPPs can drastically modify the translocation mechanism. For example, polyarginine (polyR), the simplest prototypical CPP, can induce the cell membrane pore formation. Hydrophobic amino acids create positive curvature by inserting into the membrane. Arginine simultaneously creates positive and negative curvatures, whereas lysine creates negative curvature along one direction only. This implies a compensatory relation between arginines and lysines/hydrophobes.

Why is the hydrophobic content of the TAT peptide relatively low if hydrophobicity can help generate negative Gaussian curvature? CPPs use less hydrophobic residues to generate saddle-splay curvature. This difference in sequences can potentially only induce transient pore-like translocation structures in the membrane and thus lead to shorter pore lifetimes for CPPs. Because of the amino acid composition for CPPs, the TAT peptide can mediate endocytosis with or without receptors.

Order your cell penetrating peptide now: http://lifetein.com/peptide_synthesis_services.html

The following table shows a selection of currently known CPPs, their origins and sequences.

Name

Origin

Sequence
Tat family
Tat (48-60)

HIV-1 protein

GRKKRRQRRRPPQQ
     Oligoarginine

Tat derivative

Rn
Penetralia family
     p-Antp

Antermapedia homeodomain

RQIKIWFQNRRMKWKK
     plsl

Igl-1 homeodomain

RVIRVWFQNKRCKDKK
Chimeric CPPs
     Transportan

Galanin-mastoparan

GWTLNSAGYLLGKINLKALAALAKKIL
MPG peptides
     P-beta

gp41-SV40

GALFLGFLGAAGSTMGAWSQPKKKRKV
     P-alpha

gp41-SV40

GALFLAFLAAALSLMGLWSQPKKKRRV
Pep-1

Trp-rich motif-SV40

KETWWETWWTEWSQPKKKRRV

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Obesity Treatment: A New Peptide Drug

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Targeted Drug Melts Fat in Obese Monkeys

During the past 20 years, there has been a dramatic increase in obesity in the United States and rates remain high. In 2010, no state in the United States had a prevalence of obesity less than 20%. Approximately one in three adults and one in six children are obese. Obesity is epidemic in the United States today and a major cause of death, attributable to heart disease, cancer, and diabetes.

Despite significant efforts in the past decade, very few drugs have been successfully developed for the treatment of obese patients. Currently, only two Food and Drug Administration (FDA) approved drugs for weight loss are available in the United States: the appetite suppressant phentermine and the inhibitor of fat absorption orlistat. Orlistat (Xenical) is a weight-loss medication for long-term weight loss. This medication blocks the digestion and absorption of fat in your stomach and intestines. Other attempts to treat obesity have also predominantly focused on drugs aimed at suppressing appetite or increasing metabolism, but these efforts have been hampered by their toxic side-effects. Unfortunately, it’s common to regain weight no matter what obesity treatment methods you try.

In contrast, an MD Anderson group designed a new drug, the ligand-directed peptidomimetic CKGGRAKDC-GG-D(KLAKLAK)2 (termed adipotide), which is a synthetic peptide that triggers cell death. The drug acts on white adipose tissue. The white adipose tissue is the unhealthy type of fat that accumulates under the skin and around the abdomen.

In earlier preclinical research, obese mice lost about 30 percent of their body weight with this peptidomimetic peptide. Monkeys from three different species displayed predictable and reversible changes in renal proximal tubule function. Overall and abdominal body fat levels drop, with reversible renal side effects Weight, BMI and abdominal circumference all continued to drop for three weeks after treatment ended before slowly beginning to reverse during the fourth week of the follow-up period. Monkeys in the studies demonstrated no signs of nausea or food avoidance. The renal effect was dose-dependent, predictable and reversible. This is a potentially important finding since unpleasant side-effects have limited the use of approved drugs that reduce fat absorption in the intestines.

Together, these data in primates establish adipotide as a prototype in a new class of candidate drugs that may be useful for treating obesity in humans.

This is an alternative approach based on libraries of natural, highly structured peptides that offers new opportunities for identifying effective, specific inhibitors of protein-protein interactions. Peptide libraries constitute virtually all of the available classes of protein fold structures, providing a rich source of peptides that interact specifically and with high affinity to human proteins.

Using peptide library, the MD Anderson group is able to screen and identify those that bind to specific vascular cells among the many possible “ZIP codes” present in a human vascular map. This approach may help not only in understanding the implications of each interaction identified within the interactome but also in the development of effective drugs targeted to particular protein functions. Although peptide libraries are active in animal models, the challenge remains to demonstrate efficacy and safety in a clinical setting.

www.cdc.gov/CDCTV/ObesityEpidemic/

This video explains the many factors that have contributed to the obesity epidemic, and showcases several community initiatives taking place to prevent and reduce obesity. Obesity is a national epidemic and a major contributor to some of the leading causes of death in the U.S., including heart disease, stroke, diabetes and some types of cancer. We need to change our communities into places that strongly support healthy eating and active living.

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Peptide Antigens from Tumor Cells: Potential Peptide Vaccines for Cancer

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Many tumor cells produce peptide antigens. These may be released in the bloodstream or remain on the cell surface. Certain peptide antigens have been found to be associated with specific human cancers, such as malignant melanoma, renal cell carcinoma, breast carcinoma, prostate cancer, lung carcinoma, and colon cancer. Tumor antigens can be classified into two categories based on their pattern of expression: tumor-specific antigens (TSA) and tumor-associated antigens (TAA).

Peptide Antigens

Tumor associated peptide antigens

Tumor-associated antigens (TAAs) are mostly restricted to tumor cells, whereas tumor-specific antigens (TSAs) are unique to tumor cells. Tumor-specific antigens include all proteins produced a tumor cells that have abnormal structures due to mutations of the concerned protooncogenes or tumor suppressor genes that caused the tumor in the first place.

In contrast, the mutation of genes unrelated to the tumor formation may also lead to the synthesis of abnormal proteins. These proteins are called tumor-associated antigens because they may also be present in some normal cells. An increasing number of TAAs have been identified in cancer patients as the targets of T cells and some are being tested in clinical trials. Cytotoxic T cells (CTL) can recognize small peptides (8-10 amino acids long) that have been processed from larger proteins from tumor cells. These peptides are presented to the cytotoxic T cells in the peptide-binding clefts of MHC class I molecules on the surfaces of the tumor cells. CD4+ T cells can also recognize slightly longer peptides (12-18 amino acids), which are most likely derived from larger proteins expressed or secreted by tumor cells. These are also processed and presented in association with MHC class II molecules. These peptides may be derived from any proteins synthesized by the tumor cell (proteins found in the nucleus, cytoplasm, lysosome, or plasma membrane and proteins that are secreted).

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Peptide Synthesis for Cell-penetration Studies

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The process of introducing drugs into cells has always proved a major challenge for scientists. However, cell-penetrating peptides (CPPs) have the ability to enter a cell’s plasma membrane independent of a membrane receptor. They are usually small peptides at 10-30 residues in length. The sequences of amino acids are often positively charged. These peptides can be chemically synthesized by LifeTein.

Tat, the transcription activator of the human immunodeficiency virus type 1 (HIV-1) viral genome was shown to enter cells in a non-toxic and highly efficient manner. Tat became known as the first cell-penetrating peptide.

CPPs have demonstrated themselves to be capable of delivering biologically active cargo to the cell interior. Attached to a CPP, therapeutic cargo could be delivered to an intracellular target, thus overcoming the entry restrictions set by the plasma membrane.

There are three proposed routes of CPP entry: Model 1: The inverted micelle model. Model 2: The direct penetration (pore formation) mechanism. Model 3: An endocytic mechanism of uptake. Source: Cell-penetrating peptides and their therapeutic applications, Victoria Sebbage, BioscienceHorizons, Volume 2, Number 1, March 2009.

Cell penetrating peptides

Permeable peptides for cell penetrating

Please click here for more details for cell penetrating peptide synthesis services: http://lifetein.com/Cell_Penetrating_Peptides.html

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Synthetic Peptide Vaccine Research: Problems and Accomplishments

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On October 5, 2011, Apple’s Steve Jobs died in California at age 56, seven years after being diagnosed with pancreatic cancer. Pancreatic cancer, a malignant neoplasm of the pancreas, is the fourth most common cause of cancer death worldwide. Jobs’s death is a great loss for the technology industry, and it highlights the need for effective treatments for pancreatic and other cancers.

Immune-based cancer treatments are one emerging type of therapy, and they show great potential. Synthetic-peptide-based vaccines, which are designed to elicit T cell immunity, are also a promising approach to the prevention and treatment of both infectious diseases and malignant disorders, such as cancer. A number of peptide vaccines have been successfully used to produce antigen-specific responses in pancreatic cancer patients by targeting the differences between healthy and cancerous cells.

Mucin 1 (MUC1) is a type I transmembrane glycoprotein found on cancer cells. MUC1′s extracellular domain, which is composed of a polypeptide core with multiple tandem repeats of a 20 amino acid sequence with numerous carbohydrate chains. Peptide vaccines allow the body’s own HLA-unrestricted cytotoxic T lymphocytes (CTLs) to recognize cancerous cells. In one early study, a humoral immune response against MUC1 was also shown, and circulating antibodies against its tandem repeat peptides were detected in various cancers. These findings led researchers to recognize the potential applications of MUC1 in cancer immunotherapy. MUC1 peptide vaccines are currently in clinical trials.

Cancer cells also differ from healthy ones in their telomere-building enzymes and vascular endothelial growth factors (VEGF). A telomerase-based vaccine, known as GV1001 peptide, was found to induce a telomerase-specific immune response in 63% of evaluable patients, as measured by DTH in nonresectable pancreatic cancer. Another peptide epitope vaccine, VEGF receptor (VEGFR)2-169, has been administered alongside gemcitabine to patients with advanced pancreatic cancer. A total of 83% of patients showed a median overall survival time of 8.7 months. Phase II and III studies of this VEGFR2–169 peptide vaccine therapy are currently under way in patients with recurrent pancreatic cancer.

Peptide vaccines can even be tailored to specific patients. In one pilot study, pancreatic and colorectal cancer patients were vaccinated with K-Ras peptides containing patient-specific mutations. Of the patients who received the vaccine, 20% were still alive. Memory T-cell responses were observed in 75% of the survivors. In addition, some effort has been made to generate peptide vaccines based on the specific tumor-antigen epitopes that are most immunogenic in a given patient.

Unfortunately, the advantages that peptide vaccines offer are somewhat diminished by their lack of inherent immunogenicity, as can be seen in their clinical results, which, while encouraging, do not equal those of other types of vaccines. However, there are several ways in which the immunogenicity of peptide vaccines can be increased. Key anchor residues within the peptides, such those that bind to MHC-I molecules, can be mutated. Target peptides can be linked to more highly immunogenic peptides or to antibodies. For example, the TAT protein from HIV, which facilitates entry into cells, has been fused to antigens in order to enhance uptake by cells.

Successful clinical application of peptide vaccines requires a strong understanding of how pancreatic cancers evade immune recognition and target immune suppressor mechanisms. Although peptide vaccines constitute an attractive path for immunotherapy, there are various issues that must be addressed in order to increase the likelihood that these vaccines will preferentially stimulate high-quality CTL responses. Close examination of peptide dosage and vaccine formulation and identification of potential cryptic T cell epitopes will be key parts of any future clinical study.

Read more at:http://lifetein.com/therapeutics_peptide_applications.html
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