Custom Monoclonal Antibody Services

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A: (Cat. No. LA005) Monoclonal Antibody Service from Gene to Ascites Antibodies (22-26 weeks)

1. Include gene amplification, vector construction, protein expression in E. coli., immunization and sera collection, ELISA test.
2. Splenocyte fusions are performed from mice with the best titer. The hybridoma cells are screened by ELISA.
3. 2 of these 10 clones are selected for subcloning, isotype determination, ascites production and purification
4. Deliverables: construct vector, 1 mg protein, 50 ul pre-immune sera (1:10 dilution), 2-5 clones, 2-5 mg purified antibodies (2 clones), ELISA result (>1:32,000), isotype determination result.

B: (Cat. No. LA006) Monoclonal Antibody Service from peptide design to ascites production (20-26 weeks)

1. Include peptide design and synthesis, immunization and sera collection, ELISA test.
2. Splenocyte fusions and subclone, ascites production and purification.
3. Deliverables: 2-3 mg peptides, 50 ul pre-immune sera, 2-5 clones, 2-5 mg purified antibodies (2 clones), ELISA results (>1:32,000), isotype determination result.

C: (Cat. No. LA007) Monoclonal Antibody Service using phospho-peptide antigens (21-26 weeks)

1. Include peptide synthesis
   • Phosphopeptide: >85% purity, 10 mg, 12-22 amino acids
   • Non-phosphopeptide: >85% purity, 10 mg, 12-22 amino acids
2. Conjugation of phosphopeptide to KLH or BSA (2-3 mg); Conjugation of the nonphosphorylated peptide to BSA; Immunization and sera collection; ELISA identification; Fusion and hybridoma development; Isotype determination; Subcloning and cell cryopreservation; Ascites production and purification (2 clones)
3. Standard 21-26 weeks antibody production schedule
4. Deliverables: 50 ul pre-immune sera, 2-3 mg peptides (unmodified & phospho), 2-5 mg phospho-specific antibodies (2 clones), ELISA results (>1:32,000), Isotype determination results.

D: Additional services:

• 4-Week extension: Provide 1 immunization and 2 bleed collections for approximately 80-100ml of polyclonal antisera, $200
• Affinity purification: Affinity purification of 25ml of sera. Include the affinity column for running additional purifications, $600
• ELISA: On any bleed or purified antibody according to customers' requests, $50
• Western blot: Assay of antisera or purified antibodies against the antigens according to customers' requests, $100
• Immunochemistry: Assay of purified antibodies against the pathological slices according to customers' requests, $90

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Email: antibody@lifetein.com

Tel: 1-732-312-5852

Fax: 1-732-626-6074