Custom Monoclonal Antibody Services

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A: (Cat. No. LA005) Monoclonal antibody services from gene to ascites Antibodies

1. Gene amplification, vector construction, protein expression in E. coli., immunization and sera collection, ELISA test
2. Splenocyte fusions are performed from mice with the best titers; The hybridoma cells are screened by ELISA
3. Standard 22-26-week antibody production schedule
4. Two of these 10 clones are selected for subcloning, isotype determination, ascites production and purification
5. Deliverables: construct vector, 1 mg protein, 50 ul pre-immune sera (1:10 dilution), 2 clones, 2-5 mg purified antibodies (2 clones), ELISA results >1:32,000, isotype determination

1. Include peptide design (free) and peptide synthesis, immunization and sera collection, ELISA test.
2. Splenocyte fusions and subclone, ascites production and purification.
3. Standard 20-26-week antibody production schedule
4. Deliverables: 2-3 mg peptides, 50 ul pre-immune sera, 2 clones, 2-5 mg purified antibodies (2 clones), ELISA results >1:32,000, isotype determination

C: (Cat. No. LA007) Monoclonal antibody services using phospho-peptide antigens

1. Peptide antigen design (free) and peptide synthesis
   • Phosphopeptide: >85% purity, 10 mg, 12-22 amino acids
   • Non-phosphopeptide: >85% purity, 10 mg, 12-22 amino acids
2. Conjugation of phosphopeptide to KLH or BSA (2-3 mg); conjugation of the non-phosphorylated peptide to BSA; immunization and sera collection; ELISA identification; fusion and hybridoma development; isotype determination; subcloning and cell cryopreservation; ascites production and purification (2 clones)
3. Standard 21-26 week antibody production schedule
4. Deliverables: 50 ul pre-immune sera, 2-3 mg peptides (unmodified and phospho), 2-5 mg phospho-specific antibodies (2 clones), ELISA results >1:32,000, isotype determination

D: Additional services:

• 4-week extension: We provide 1 additional immunization and 2 additional bleed collections for approximately 80-100ml of polyclonal antisera
• Affinity purification: Affinity purification of 25ml of sera including the affinity column for running additional purifications
• ELISA: On any bleed or purified antibody, upon request
• Western blot: Assay of antisera or purified antibodies against the antigens, upon request
• Immunochemistry: Assay of purified antibodies against the pathological slices, upon request

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Email: antibody@lifetein.com

Tel: 1-888-791-1618

Fax: 1-888-791-1618