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| Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit mAb |
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| LTA24832 |
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| 100ul |
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$750
In stock
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| Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. |
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| S6; eS6 |
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| 6194,sc-13005,sc-13007,sc-20085,40S ribosomal protein S6,Phosphoprotein NP33,ribosomal protein S6,RPS6,S6,phosphoprotein NP33,small ribosomal subunit protein eS6,P62753,Ribosomal Protein S6,Small Ribosomal Subunit Protein ES6,40S Ribosomal Protein S6,RPS6 |
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| Human |
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| STSKS |
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| A synthetic phosphorylated peptide around S240 & S244 of human RPS6 (NP_001001.2). |
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| Rabbit |
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| Affinity Purified Monoclonal IgG |
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| WB, ELISA |
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| Human, Mouse, Rat |
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| 29kDa |
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| WB,1:54000 - 1:162000|ELISA,Recommended starting concentration is 1 _g/mL. Please optimize the concentration based on your specific assay requirements. |
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Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.
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Shipped at ambient temperature.
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Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
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| .jpg )
Western blot analysis of various lysates using Phospho-S6 Ribosomal Protein (RPS6)-S240/244 Rabbit mAb (LTA24832) at 1:54000 dilution incubated overnight at 4_. HeLa,NIH/3T3 and PC-12 cells were treated by 10% FBS at 37_ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 30 _g per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (LT00020).|Exposure time: 30s. |
