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| Phospho-PPP1CA-T320 Rabbit mAb |
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| LTA24834 |
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| 100ul |
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$750
In stock
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| The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). This broadly expressed gene encodes the alpha subunit of the PP1 complex that associates with over 200 regulatory proteins to form holoenzymes which dephosphorylate their biological targets with high specificity. PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies suggest that PP1 is an important regulator of cardiac function and that PP1 deregulation is implicated in diabetes and multiple types of cancer. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
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| PP1A; PP-1A; PPP1A; PP1alpha |
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| 5499,sc-6105,sc-6104,sc-443,PPP1CA,PP-1A,PP1A,PP1alpha,PPP1A,protein phosphatase 1 catalytic subunit alpha,serine/threonine-protein phosphatase PP1-alpha catalytic subunit,protein phosphatase 1,catalytic subunit,alpha isoform,alpha isozyme,serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit,testicular tissue protein Li 155,P62136,Protein Phosphatase 1 Catalytic Subunit Alpha,Protein Phosphatase 1,Catalytic Subunit,Alpha Isoform,Alpha Isozyme,Serine/Threonine Protein Phosphatase PP1-Alpha 1 Catalytic Subunit,Serine/Threonine-Protein Phosphatase PP1-Alpha Catalytic Subunit,Testicular Tissue Protein Li 155,EC 3.1.3.16 |
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| Human |
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| PITPP |
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| A synthetic phosphorylated peptide around T320 of human PPP1CA (NP_002699.1). |
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| Rabbit |
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| Affinity Purified Monoclonal IgG |
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| WB, ELISA |
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| Human, Mouse, Rat |
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| 38kDa |
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| WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 _g/mL. Please optimize the concentration based on your specific assay requirements. |
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Reconstituted antibodies stable at -80C for 12 months, 4C for 1 week. Avoid repeated freeze-thaw cycles.
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Shipped at ambient temperature.
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Centrifuge tubes before opening. Dissolve the lyophilized antibodies in distilled water. 5-10% glycerol is recommended.
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| .jpg )
Western blot analysis of lysates from C6 cells using Phospho-PPP1CA-T320 Rabbit mAb (LTA24834) at 1:1000 dilution incubated overnight at 4_. C6 cells were treated by Calyculin A (100 nM) at 37_ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (LT014) at 1:10000 dilution.|Lysates/proteins: 30 _g per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit (LT00020).| Exposure time: 60s. |
