HRP conjugated Anti-human β-Actin Monoclonal Ab (clone 4C4)

Catalog Number:
LT_R002
Quantity:
50ug
Immunogen:
Full length recombinant human β-actin protein
IgG type:
Rabbit IgG
Clonality:
Monoclonal
Applications:
ELISA, WB
Specificity:
β-actin from human, mouse, rat, pig
Formulation:
0.05 M carbonate-bicarbonate buffer, 50% glycerol, pH 9.4
Purity:
> 95% determined by SDS-PAGE
Storage:

This antibody is shipped at 4 °C. This product is stable for 12 months from date of receipt when stored at -20 °C to -70 °C. Avoid freeze/thaw cycles.

Preparation:

Monoclonal antibody is produced by immunizing rabbit with full length human β-actin and purified using protein A resin.

Hazard/Biohazard:

This antibody contains 0.09% sodium azide as preservative. Please handle and dispose the product properly. No known biohazard is associated with this product.

Background:

β-actin is a member of highly conserved actins that are involved in cell motility, structure, and integrity. β-actin exists in two forms in cytoplasm: globular actin monomer (G-actin) with molecular weight approximately 42 kDa and fibrous polymer (F-actin). Due to its ubiquitous expression in eukaryotic cells, β-actin is widely used as a loading control for western blotting, markers for the integrity of cells. Defects in β-actin are a cause of dystonia juvenile-onset (DYTJ), often leading to abnormal postures, dopa-unresponsive
dystonia, developmental malformations, and hearing loss. Research studies have also demonstrated that actin is associated with malignancies such as breast cancer and lymphoma.

Images:


Fig. 1 Western blot

Western blot conditions
Lanes:
1. HELA (human)
2. EL4.BU.1.OUAr.1.1 (mouse)
Each lane was loaded with 10 ug cell lysate.
mAb dilution: 1:1000
Blocking and antibody dilution buffer is 5% skim milk (w/v), 1x TBS, 0.05% Tween-20.

  • 4 Units in Stock

$250.00

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