|
LT_R004 |
| 50ug |
|
Full length native human
CKMB protein |
| Rabbit IgG |
|
Monoclonal |
|
TIA, ELISA |
|
Human CKMB |
|
0.22 μM filtered solution of PBS,
pH 7.4 |
| > 95% determined by SDS-PAGE |
|
This antibody is shipped at 4 °C. This product is stable for
12 months from date of receipt when stored at -20 °C to -70
°C. Avoid freeze/thaw cycles. |
|
Monoclonal antibody is produced by immunizing rabbit with
full length human CKMB and purified using protein A resin. |
|
This antibody contains 0.09% sodium azide as preservative.
Please handle and dispose the product properly. No
known biohazard is associated with this product. |
|
Creatine kinase (CK) is a dimeric enzyme expressed by
diverse tissues and cell types with calculated molecular
weight of 86 KD for dimer and 43 KD for monomer. CK
converts creatine to phosphocreatine by consuming ATP. It
has two distinct types of subunits CKM (muscle type) and
CKB (brain type), generating three combinations: CKMM,
CKMB, CKBB. CKBB is found in brain and smooth muscle
as well as in other tissues and cells such as neuronal cells,
retina, kidney, and bone. CKMM is predominantly expressed
in skeletal and cardiac muscles. CKMB heterodimer is found
prominently in heart and released upon myocardial infarction.
CKMB is an important serum marker for myocardial
infarction.
|
|
Fig. 1 ELISA
ELISA conditions
1) Antigen: human CKMB
protein at 1.0 μg/ml
2) detection antibody: rabbit
anti-CKMB monoclonal
antibody (clone 8D10, LT_R004) at initial 1.0
μg/ml followed by 1:3 serial
dilutions.
|
