|
LT_R001 |
| 50ug |
|
Full length recombinant human
β-actin protein |
| Rabbit IgG |
|
Monoclonal |
|
ELISA, WB, IF |
|
β-actin from human, mouse,
rat, pig |
|
0.22 μM filtered solution of PBS,
0.09% NaN3, 50% glycerol, pH 7.4 |
| > 95% determined by SDS-PAGE |
|
This antibody is shipped at 4 °C. This product is stable for
12 months from date of receipt when stored at -20 °C to -70
°C. Avoid freeze/thaw cycles. |
|
Monoclonal antibody is produced by immunizing rabbit with
full length human β-actin and purified using protein A resin. |
|
This antibody contains 0.09% sodium azide as preservative.
Please handle and dispose the product properly. No
known biohazard is associated with this product. |
|
β-actin is a member of highly conserved actins that are
involved in cell motility, structure, and integrity. β-actin exists
in two forms in cytoplasm: globular actin monomer (G-actin)
with molecular weight approximately 42 kDa and fibrous
polymer (F-actin). Due to its ubiquitous expression in
eukaryotic cells, β-actin is widely used as a loading control
for western blotting, markers for the integrity of cells. Defects
in β-actin are a cause of dystonia juvenile-onset (DYTJ),
often leading to abnormal postures, dopa-unresponsive
dystonia, developmental malformations, and hearing loss.
Research studies have also demonstrated that actin is associated
with malignancies such as breast cancer and lymphoma. |
|
Fig. 1 Western blot
Western blot conditions
Lanes:
1. HELA (human)
2. EL4.BU.1.OUAr.1.1 (mouse)
Each lane was loaded with 10 ug cell
lysate.
mAb dilution: 1:1000
Blocking and antibody dilution buffer
is 5% skim milk (w/v), 1x TBS,
0.05% Tween-20.
|
