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Peptide FAQ

Frequently Asked Peptide Questions
How long do peptides last in the fridge? How should peptides be stored? For the ...
how to perform Immunohistochemistry Using Biotinylated Peptide?
A peptide RK-10 is used to detect Programmed Death Ligand 1 (PD-L1) expressing t...
How to prepare the Cy3 and Cy5 labeled peptides?
* Peptides were synthesized using standard Fmoc solid-phase peptide synthesis. ...
How to perform Peptide–oligonucleotide conjugation?
1. 10 nmol (20 µl, 500 µM) of oligonucleotide was mixed with 60 µl of 100 mM pot...
How to perform competitive ELISA?
1.ELISA plates were coated with 100 μl of the mutant peptide (200 ng/ml) overnig...
How to do epitope mapping of monoclonal antibodies using overlapping peptides?
1.Mapping of the monoclonal antibody epitopes was performed using linear peptide...
How to remove TFA from synthetic peptides using HCl?
It is important that purified peptides be free of Trifluoroacetate (TFA) because...
Strategies for the Synthesis of Labeled Peptides
Labeled peptides are frequently used by researchers for binding studies, to dete...
How can I improve the purity of my protein without reducing the yield?
Q How can I improve protein purity without reducing yield? I encountered a probl...
Synthesis of multiple antigenic peptides
Synthesis of multiple antigenic peptides: strategies and limitations Dendrimeri...
Peptide-protein interactions
Did you know that 15%–40% of all interactions in the cell are mediated through p...
Peptide smells
Synthetic peptides using a large quantities of DMS and thiol scavengers will pro...