{"id":624,"date":"2019-12-17T14:43:32","date_gmt":"2019-12-17T19:43:32","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=624"},"modified":"2024-11-16T21:37:20","modified_gmt":"2024-11-17T02:37:20","slug":"new-publication-cell-cited-lifetein-biotinylated-peptide-products","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/new-publication-cell-cited-lifetein-biotinylated-peptide-products\/","title":{"rendered":"New Publication: Cell Cited LifeTein Biotinylated Peptide Products"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"272\" src=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/12\/Screen-Shot-2019-12-17-at-2.41.48-PM-1024x272.png\" alt=\"\" class=\"wp-image-625\" srcset=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/12\/Screen-Shot-2019-12-17-at-2.41.48-PM-1024x272.png 1024w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/12\/Screen-Shot-2019-12-17-at-2.41.48-PM-300x80.png 300w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/12\/Screen-Shot-2019-12-17-at-2.41.48-PM-768x204.png 768w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/12\/Screen-Shot-2019-12-17-at-2.41.48-PM-500x133.png 500w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/12\/Screen-Shot-2019-12-17-at-2.41.48-PM.png 1068w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">Pull-down assay using biotinylated peptides<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">Giantin, a novel conserved Golgi membrane protein, is a disulfide-linked homodimer. It was found that BFA-induced Golgi disorganization is associated with the monomerization of giantin.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The pull-down experiment was performed. The control peptide biotin-GHGTGSTGSGSMLRTLLRRRL synthesized by LifeTein was incubated with lysate and Dynabeads, as well as the lysate incubated with Dynabeads only served as a control. Dynabeads carrying MGAT1 peptide were able to pull-down giantin from the lysate of HeLa cells, however, giantin was not detected in the pull-down fraction from the lysate exposed to the Dynabeads or in combination with control peptide. It is logical to hypothesize that the MGAT1 binding domain of giantin lies within its N-terminal non-coiled-coil area. <\/p>\n\n\n\n<p class=\"wp-block-paragraph\">The Dynabeads function similarly to LifeTein magnetic beads:<br \/>\nhttps:\/\/www.lifetein.com\/peptide-product\/amineactivated-peptide-conjugation-magnetic-beads-p-3647.html<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Cells 2019, 8(12), 1631; https:\/\/doi.org\/10.3390\/cells8121631<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Giantin, a novel conserved Golgi membrane protein, is a disulfide-linked homodimer. It was found that BFA-induced Golgi disorganization is associated with the monomerization of giantin. The pull-down experiment was performed. The control peptide biotin-GHGTGSTGSGSMLRTLLRRRL synthesized by LifeTein was incubated with &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/new-publication-cell-cited-lifetein-biotinylated-peptide-products\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-624","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/624","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=624"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/624\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/625"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=624"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=624"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=624"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}