{"id":567,"date":"2019-06-18T10:28:27","date_gmt":"2019-06-18T14:28:27","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=567"},"modified":"2024-11-16T21:44:32","modified_gmt":"2024-11-17T02:44:32","slug":"how-to-identify-a-pd-l1-binding-peptide-for-determination-of-pdl1-expression-in-tumors","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/how-to-identify-a-pd-l1-binding-peptide-for-determination-of-pdl1-expression-in-tumors\/","title":{"rendered":"How to Identify a PD-L1 Binding Peptide for Determination of PDL1 Expression in Tumors"},"content":{"rendered":"\n
\"\"
PD-L1 Binding Peptides<\/figcaption><\/figure>\n\n\n\n

Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. <\/p>\n\n\n\n

    \n
  1. Identifying the PD-L1 binding peptide and mock peptide: Select from a library of peptides for stability and high affinity.<\/li>\n\n\n\n
  2. Fluorescent target peptide-Cy5 as Marker for Flow Cytometry.<\/li>\n\n\n\n
  3. Detection of PD-L1 in circulating tumor cells using the Cy5 or Cy7 (infrared) labeled peptides. <\/li>\n\n\n\n
  4. Biotinylated peptides to detect PD-L1 in sample tissues. The streptavidin-HRP are used.<\/li>\n\n\n\n
  5. Fluorescent Cy5-labeled peptides detects PD-L1 in sample tissues. <\/li>\n<\/ol>\n\n\n\n

    By utilizing a peptide-based approach, it is possible to detect all levels of PD-L1 with high sensitivity and specificity.<\/p>\n\n\n\n

    Reference:<\/p>\n\n\n\n

    https:\/\/www.nature.com\/articles\/s41598-017-10946-2<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. By utilizing a peptide-based approach, it is possible to detect all levels of PD-L1 with high sensitivity … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":568,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-567","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"Peptide for Determination of PDL1 Expression in Tumors | LifeTein Peptide Blog","description":"Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. 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