{"id":567,"date":"2019-06-18T10:28:27","date_gmt":"2019-06-18T14:28:27","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=567"},"modified":"2024-11-16T21:44:32","modified_gmt":"2024-11-17T02:44:32","slug":"how-to-identify-a-pd-l1-binding-peptide-for-determination-of-pdl1-expression-in-tumors","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/how-to-identify-a-pd-l1-binding-peptide-for-determination-of-pdl1-expression-in-tumors\/","title":{"rendered":"How to Identify a PD-L1 Binding Peptide for Determination of PDL1 Expression in Tumors"},"content":{"rendered":"\n<figure class=\"wp-block-image\"><img decoding=\"async\" width=\"1024\" height=\"501\" src=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/06\/PD1-tumor-peptides-1024x501.png\" alt=\"\" class=\"wp-image-568\" srcset=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/06\/PD1-tumor-peptides-1024x501.png 1024w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/06\/PD1-tumor-peptides-300x147.png 300w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/06\/PD1-tumor-peptides-768x376.png 768w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/06\/PD1-tumor-peptides-500x245.png 500w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2019\/06\/PD1-tumor-peptides.png 1728w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">PD-L1 Binding Peptides<\/figcaption><\/figure>\n\n\n\n<p class=\"wp-block-paragraph\">Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. <\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Identifying the PD-L1 binding peptide and mock peptide: Select from a library of peptides for stability and high affinity.<\/li>\n\n\n\n<li>Fluorescent target peptide-Cy5 as Marker for Flow Cytometry.<\/li>\n\n\n\n<li>Detection of PD-L1 in circulating tumor cells using the Cy5 or Cy7 (infrared) labeled peptides. <\/li>\n\n\n\n<li>Biotinylated peptides to detect PD-L1 in sample tissues. The streptavidin-HRP are used.<\/li>\n\n\n\n<li>Fluorescent Cy5-labeled peptides detects PD-L1 in sample tissues. <\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\">By utilizing a peptide-based approach, it is possible to detect all levels of PD-L1 with high sensitivity and specificity.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Reference:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.nature.com\/articles\/s41598-017-10946-2\">https:\/\/www.nature.com\/articles\/s41598-017-10946-2<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Blocking the interaction between Programmed Death Ligand 1 (PD-L1) and its receptor, PD-1, is an effective method of treating many types of cancers. By utilizing a peptide-based approach, it is possible to detect all levels of PD-L1 with high sensitivity &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/how-to-identify-a-pd-l1-binding-peptide-for-determination-of-pdl1-expression-in-tumors\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":568,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_crdt_document":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-567","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/567","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=567"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/567\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/568"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=567"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=567"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=567"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}