{"id":565,"date":"2019-05-28T18:25:18","date_gmt":"2019-05-28T22:25:18","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=565"},"modified":"2024-11-16T21:47:32","modified_gmt":"2024-11-17T02:47:32","slug":"how-to-screen-small-peptide-ligands-for-targeting-receptors-in-cancer-cells","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/how-to-screen-small-peptide-ligands-for-targeting-receptors-in-cancer-cells\/","title":{"rendered":"How to screen small peptide ligands for targeting receptors in cancer cells?"},"content":{"rendered":"\n
\"Neoepitopes
Neoepitopes as cancer immunotherapy targets<\/figcaption><\/figure>\n\n\n\n
    \n
  1. Design a biotin-labeled peptide library: overlapping or Alanine scan<\/li>\n\n\n\n
  2. The peptide library is coated on the avidin-coated 96 well plates.<\/li>\n\n\n\n
  3. The Peptide library is used to evaluate the binding of each peptide to different cell lines using CyQUANT Cell Proliferation Assays.<\/li>\n\n\n\n
  4. Flow cytometry: Determine the cellular uptake of selected FITC-labeled peptides.<\/li>\n\n\n\n
  5. Competitive assay: The cells are incubated with FITC-peptide with or without excess unlabeled peptide (100-fold)<\/li>\n\n\n\n
  6. Finetune the peptide sequence: structure analysis, cyclization, and D amino acid modification. <\/li>\n\n\n\n
  7. Find the promising receptor-specific cancer cell binding peptide that can be conjugated directly to a chemotherapeutic drug or to nanoparticles for targeted drug delivery to enhance the efficacy of chemotherapy for cancer treatment.<\/li>\n<\/ol>\n\n\n\n

    Reference: <\/p>\n\n\n\n

    https:\/\/www.nature.com\/articles\/s41598-019-38574-y<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Reference: https:\/\/www.nature.com\/articles\/s41598-019-38574-y<\/p>\n","protected":false},"author":1,"featured_media":508,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-565","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"How to screen small peptide ligands for targeting receptors in cancer cells? | LifeTein Peptide Blog","description":"Find the promising receptor specific cancer cell binding peptide that can be conjugated directly to a chemotherapeutic drug or to nanoparticles for targeted drug delivery to enhance the efficacy of chemotherapy for cancer treatment.","canonical_url":"https:\/\/www.lifetein.com\/blog\/how-to-screen-small-peptide-ligands-for-targeting-receptors-in-cancer-cells\/","robots":"max-image-preview:large","keywords":"peptide library,peptide drug","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"article","og:title":"How to screen small peptide ligands for targeting receptors in cancer cells? 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