{"id":565,"date":"2019-05-28T18:25:18","date_gmt":"2019-05-28T22:25:18","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=565"},"modified":"2024-11-16T21:47:32","modified_gmt":"2024-11-17T02:47:32","slug":"how-to-screen-small-peptide-ligands-for-targeting-receptors-in-cancer-cells","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/how-to-screen-small-peptide-ligands-for-targeting-receptors-in-cancer-cells\/","title":{"rendered":"How to screen small peptide ligands for targeting receptors in cancer cells?"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"1024\" height=\"527\" src=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/09\/Neoepitopes-as-cancer-immunotherapy-targets-key-challenges-and-opportunities-1024x527.jpg\" alt=\"Neoepitopes as cancer immunotherapy targets: key challenges and opportunities\" class=\"wp-image-508\" srcset=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/09\/Neoepitopes-as-cancer-immunotherapy-targets-key-challenges-and-opportunities-1024x527.jpg 1024w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/09\/Neoepitopes-as-cancer-immunotherapy-targets-key-challenges-and-opportunities-300x155.jpg 300w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/09\/Neoepitopes-as-cancer-immunotherapy-targets-key-challenges-and-opportunities-768x396.jpg 768w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/09\/Neoepitopes-as-cancer-immunotherapy-targets-key-challenges-and-opportunities-500x258.jpg 500w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/09\/Neoepitopes-as-cancer-immunotherapy-targets-key-challenges-and-opportunities.jpg 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"wp-element-caption\">Neoepitopes as cancer immunotherapy targets<\/figcaption><\/figure>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Design a biotin-labeled peptide library: overlapping or Alanine scan<\/li>\n\n\n\n<li>The peptide library is coated on the avidin-coated 96 well plates.<\/li>\n\n\n\n<li>The Peptide library is used to evaluate the binding of each peptide to different cell lines using CyQUANT Cell Proliferation Assays.<\/li>\n\n\n\n<li>Flow cytometry: Determine the cellular uptake of selected FITC-labeled peptides.<\/li>\n\n\n\n<li>Competitive assay: The cells are incubated with FITC-peptide with or without excess unlabeled peptide (100-fold)<\/li>\n\n\n\n<li>Finetune the peptide sequence: structure analysis,  cyclization, and D amino acid modification. <\/li>\n\n\n\n<li>Find the promising receptor-specific cancer cell binding peptide that can be conjugated directly to a chemotherapeutic drug or to nanoparticles for targeted drug delivery to enhance the efficacy of chemotherapy for cancer treatment.<\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\">Reference: <\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.nature.com\/articles\/s41598-019-38574-y\">https:\/\/www.nature.com\/articles\/s41598-019-38574-y<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Reference: https:\/\/www.nature.com\/articles\/s41598-019-38574-y<\/p>\n","protected":false},"author":1,"featured_media":508,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_crdt_document":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[],"class_list":["post-565","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/565","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=565"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/565\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/508"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=565"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=565"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=565"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}