The folate receptor alpha (FR\u03b1) is highly expressed in ovarian cancer and not in normal tissues. An FR\u03b1 binding peptide C7 (Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser, MHTAPGWGYRLS)\u00a0 was found to bind to FR\u03b1 expressing cells. This tumor-targeting peptide was proved by both phage homing experiment and fluorescence imaging.<\/p>\n
Tumor Targeting of Conjugated Synthetic Peptides
\n1. The FITC-conjugated peptide FITC-MHTAPGWGYRLS was dissolved in PBS.
\n2. The peptide was injected intravenously into a tumor-bearing nude mouse.
\n3. After 2 h, the tumor and other organ tissues were harvested and analyzed using a fluorescence imaging system.<\/p>\n
Cell internalization of Synthesized Peptide
\n1. Cells were seeded in 24 well plates containing coverslips and incubated for 24 hours in a medium with FBS.
\n2. FITC conjugated peptide was incubated with the cells for 4 hours at 37 \u00b0C.
\n3. The cells were washed once with PBS and fixed in 4% paraformaldehyde.
\n4. Cells were washed three times with PBS and stained with DAPI for 20 min at room temperature.
\n5. Internalized fluorescent signals were imaged with a confocal microscope.<\/p>\n
This tumor-specific peptide could be a potent and selective ligand for FR\u03b1. It has a great potential for the delivery of cancer therapeutics or imaging agents to express tumors.<\/p>\n
Reference: Scientific Reports, volume 8, Article number: 8426 (2018)<\/p>\n
![\"tumor](\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/07\/Tumor-targeting-peptides-300x269.jpg\")
tumor targeting peptides<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"
The folate receptor alpha (FR\u03b1) is highly expressed in ovarian cancer and not in normal tissues. An FR\u03b1 binding peptide C7 (Met-His-Thr-Ala-Pro-Gly-Trp-Gly-Tyr-Arg-Leu-Ser, MHTAPGWGYRLS)\u00a0 was found to bind to FR\u03b1 expressing cells. This tumor-targeting peptide was proved by both phage homing … Continue reading