{"id":483,"date":"2018-06-26T17:55:56","date_gmt":"2018-06-26T21:55:56","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=483"},"modified":"2024-11-16T22:01:44","modified_gmt":"2024-11-17T03:01:44","slug":"how-to-analyze-the-cell-viability-with-peptides","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/how-to-analyze-the-cell-viability-with-peptides\/","title":{"rendered":"How to analyze the cell viability with peptides?"},"content":{"rendered":"
    \n
  1. Cells were seeded on 96-well culture plates (10000 cells\/well) and incubated in 100 \u03bcL of DMEM containing 10% FBS.<\/li>\n
  2. The medium was then replaced with fresh medium containing 10% FBS, and a peptide solution was added to each well at an appropriate concentration (for example 0.5uM, 1uM, 1.5uM, 2uM).<\/li>\n
  3. After a 2-h incubation, Cell counting kit-8 (CCK-8) was used according to the manufacturer\u2019s protocol. Cell Counting Kit-8 allows sensitive colorimetric assays for determining cell viability in cell proliferation and cytotoxicity assays.<\/li>\n
  4. Cell viability was evaluated by the absorbance of formazan from each well, and 100% cell viability was calculated from the wells without peptides.<\/li>\n
  5. The results are the mean and standard deviation obtained from 5 samples.<\/li>\n<\/ol>\n
    \"\"

    cell-viability<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

    Cells were seeded on 96-well culture plates (10000 cells\/well) and incubated in 100 \u03bcL of DMEM containing 10% FBS. The medium was then replaced with fresh medium containing 10% FBS, and a peptide solution was added to each well at … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":475,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[125,124],"class_list":["post-483","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-cell-viability","tag-cytotoxicity"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"How to analyze the cell viability with peptides? | LifeTein Peptide Blog","description":"Peptides were added to cells for cell viability test. The protocol of cell viability study is presented. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.","canonical_url":"https:\/\/www.lifetein.com\/blog\/how-to-analyze-the-cell-viability-with-peptides\/","robots":"max-image-preview:large","keywords":"cell viability,peptide cell incubation,peptide cytotoxicity,cytotoxicity","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"article","og:title":"How to analyze the cell viability with peptides? | LifeTein Peptide Blog","og:description":"Peptides were added to cells for cell viability test. The protocol of cell viability study is presented. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.","og:url":"https:\/\/www.lifetein.com\/blog\/how-to-analyze-the-cell-viability-with-peptides\/","fb:admins":"233983946638783","og:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","og:image:secure_url":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","article:tag":["cell viability","peptide cell incubation","peptide cytotoxicity","cytotoxicity","peptide"],"article:published_time":"2018-06-26T21:55:56+00:00","article:modified_time":"2024-11-17T03:01:44+00:00","article:publisher":"https:\/\/www.facebook.com\/LifeTein-LLC-233983946638783\/","twitter:card":"summary_large_image","twitter:site":"@lifetein","twitter:title":"How to analyze the cell viability with peptides? | LifeTein Peptide Blog","twitter:description":"Peptides were added to cells for cell viability test. The protocol of cell viability study is presented. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.","twitter:creator":"@lifetein","twitter:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg"},"aioseo_meta_data":{"post_id":"483","title":"How to analyze the cell viability with peptides? | #site_title","description":"Peptides were added to cells for cell viability test. The protocol of cell viability study is presented. Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.","keywords":[{"label":"cell viability","value":"cell viability"},{"label":"peptide cell incubation","value":"peptide cell incubation"},{"label":"peptide cytotoxicity","value":"peptide cytotoxicity"}],"keyphrases":{"focus":{"keyphrase":"peptide for cell study","score":37,"analysis":{"keyphraseInTitle":{"score":3,"maxScore":9,"error":1},"keyphraseInDescription":{"score":3,"maxScore":9,"error":1},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":4},"keyphraseInURL":{"score":1,"maxScore":5,"error":1},"keyphraseInIntroduction":{"score":3,"maxScore":9,"error":1},"keyphraseInSubHeadings":[],"keyphraseInImageAlt":{"score":3,"maxScore":9,"error":1},"keywordDensity":{"score":0,"type":"low","maxScore":9,"error":1}}},"additional":[]},"primary_term":null,"canonical_url":null,"og_title":null,"og_description":null,"og_object_type":"article","og_image_type":"default","og_image_url":null,"og_image_width":null,"og_image_height":null,"og_image_custom_url":null,"og_image_custom_fields":null,"og_video":"","og_custom_url":null,"og_article_section":null,"og_article_tags":null,"twitter_use_og":true,"twitter_card":"summary_large_image","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":[],"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"Article","isEnabled":true},"graphs":[]},"schema_type":null,"schema_type_options":null,"pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":"-1","robots_max_videopreview":"-1","robots_max_imagepreview":"none","priority":null,"frequency":"default","location":null,"local_seo":null,"seo_analyzer_scan_date":null,"breadcrumb_settings":null,"limit_modified_date":false,"open_ai":"{\"title\":{\"suggestions\":[],\"usage\":0},\"description\":{\"suggestions\":[],\"usage\":0}}","ai":null,"created":"2020-12-04 22:27:30","updated":"2025-06-18 02:47:18"},"aioseo_breadcrumb":"

    \n\t\t\tHome<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tPeptide<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tHow to analyze the cell viability with peptides?\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/www.lifetein.com\/blog"},{"label":"Peptide","link":"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/"},{"label":"How to analyze the cell viability with peptides?","link":"https:\/\/www.lifetein.com\/blog\/how-to-analyze-the-cell-viability-with-peptides\/"}],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/483","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=483"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/483\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/475"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=483"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=483"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=483"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}