{"id":468,"date":"2018-05-18T16:12:32","date_gmt":"2018-05-18T20:12:32","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=468"},"modified":"2024-11-16T22:05:19","modified_gmt":"2024-11-17T03:05:19","slug":"trans-activator-of-transcription-tat-with-nuclear-translocation-signal-peptide-drug-candidate","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/trans-activator-of-transcription-tat-with-nuclear-translocation-signal-peptide-drug-candidate\/","title":{"rendered":"New Drug Candidate: Tat Protein with Nuclear Translocation Signal Peptide"},"content":{"rendered":"
\"Endocytosis

Endocytosis TAT Peptide<\/p><\/div>\n

Nuclear translocation of annexin A1 (ANXA1) has been reported to participate in diverse cellular processes. It was found that the amino-acid residues from R228 to F237 function as a unique nuclear translocation signal (NTS) and are required for nuclear translocation of ANXA1.<\/p>\n

Transactivator of transcription (Tat) is a cell-penetrating peptide. With high efficiency, the peptide can translocate numerous proteins, peptides, DNA, RNA, and small drugs into the cytoplasm. The Tat-NTS (NH2-YGRKKRRQRRR-RSFPHLRRVF-CONH2) peptide was found to block the interaction of ANXA1 with importing, specifically \u03b2. This blocking would inhibit the nuclear translocation of ANXA1, consequently protecting neurons from ischemic stroke damage. So, the Tat-NTS peptide can be used as a novel and potentially promising new therapeutic candidate for the treatment of ischemic stroke.<\/p>\n

From the experiment, the Tat-NTS peptide-treated mice displayed remarkable cognitive improvement. In addition, the Tat-NTS peptide had no noticeable effect on neuronal apoptosis. So the administration of Tat-NTS peptide to dissociation of ANXA1\u2013importin \u03b2 interaction may be a new drug in ischemic stroke therapy.<\/p>\n

The Tat sequence is originally from a crucial non-structural protein of human immunodeficiency virus (HIV). The function of Tat is to bind to the viral long terminal repeat (LTR) and activate cellular transcription machinery to initiate transcription of the viral proteins. Later, this 11-amino acid positively charged peptide was found to pull diverse molecules across cell membranes in vitro and in vivo. Fusion proteins constructed with TAT rapidly enter and exit cells and cross intracellular membranes. Electrostatic interactions between TAT and the cell membrane have been implicated as a part of the transduction mechanism. Neither apoptosis nor necrosis is induced in cells after exposure to TAT.<\/p>\n

https:\/\/www.nature.com\/articles\/s41418-018-0116-5<\/p>\n","protected":false},"excerpt":{"rendered":"

Nuclear translocation of annexin A1 (ANXA1) has been reported to participate in diverse cellular processes. It was found that the amino-acid residues from R228 to F237 function as a unique nuclear translocation signal (NTS) and are required for nuclear translocation … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":487,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[69,113,112,114],"class_list":["post-468","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-cell-penetration-peptide","tag-nuclear-translocation-signal","tag-tat","tag-trans-activator-of-transcription"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"Trans-activator of transcription (Tat) with nuclear translocation signal peptide drug candidate | LifeTein Peptide Blog","description":"The Tat sequence is originally from a crucial non-structural protein of human immunodeficiency virus (HIV). This 11-amino acid positively charged TAT peptide can pull diverse molecules across cell membranes in vitro and in vivo. Trans-activator of transcription (Tat) is a cell-penetrating peptide. The peptide can translocate numerous proteins, peptides, DNA, RNA, and small drugs into the cytoplasm with high efficiency. The Tat-NTS (NH2-YGRKKRRQRRR-RSFPHLRRVF-CONH2) peptide was found to specifically block the interaction of ANXA1 with importin \u03b2. This blocking would inhibit the nuclear translocation of ANXA1, consequently protecting neurons from ischemic stroke damage. So the Tat-NTS peptide can be used as a novel and potentially promising new therapeutic candidate for the treatment of ischemic stroke.","canonical_url":"https:\/\/www.lifetein.com\/blog\/trans-activator-of-transcription-tat-with-nuclear-translocation-signal-peptide-drug-candidate\/","robots":"max-image-preview:large","keywords":"tat,cpp,trans-activator of transcription,nuclear translocation signal,peptide synthesis,cell penetration peptide","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"article","og:title":"Trans-activator of transcription (Tat) with nuclear translocation signal peptide drug candidate | LifeTein Peptide Blog","og:description":"The Tat sequence is originally from a crucial non-structural protein of human immunodeficiency virus (HIV). This 11-amino acid positively charged TAT peptide can pull diverse molecules across cell membranes in vitro and in vivo. Trans-activator of transcription (Tat) is a cell-penetrating peptide. The peptide can translocate numerous proteins, peptides, DNA, RNA, and small drugs into the cytoplasm with high efficiency. The Tat-NTS (NH2-YGRKKRRQRRR-RSFPHLRRVF-CONH2) peptide was found to specifically block the interaction of ANXA1 with importin \u03b2. This blocking would inhibit the nuclear translocation of ANXA1, consequently protecting neurons from ischemic stroke damage. So the Tat-NTS peptide can be used as a novel and potentially promising new therapeutic candidate for the treatment of ischemic stroke.","og:url":"https:\/\/www.lifetein.com\/blog\/trans-activator-of-transcription-tat-with-nuclear-translocation-signal-peptide-drug-candidate\/","fb:admins":"233983946638783","og:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","og:image:secure_url":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","article:tag":["tat","cpp","trans-activator of transcription","nuclear translocation signal","peptide synthesis","cell penetration peptide","peptide"],"article:published_time":"2018-05-18T20:12:32+00:00","article:modified_time":"2024-11-17T03:05:19+00:00","article:publisher":"https:\/\/www.facebook.com\/LifeTein-LLC-233983946638783\/","twitter:card":"summary","twitter:site":"@lifetein","twitter:title":"Trans-activator of transcription (Tat) with nuclear translocation signal peptide drug candidate | LifeTein Peptide Blog","twitter:description":"The Tat sequence is originally from a crucial non-structural protein of human immunodeficiency virus (HIV). This 11-amino acid positively charged TAT peptide can pull diverse molecules across cell membranes in vitro and in vivo. Trans-activator of transcription (Tat) is a cell-penetrating peptide. The peptide can translocate numerous proteins, peptides, DNA, RNA, and small drugs into the cytoplasm with high efficiency. The Tat-NTS (NH2-YGRKKRRQRRR-RSFPHLRRVF-CONH2) peptide was found to specifically block the interaction of ANXA1 with importin \u03b2. This blocking would inhibit the nuclear translocation of ANXA1, consequently protecting neurons from ischemic stroke damage. So the Tat-NTS peptide can be used as a novel and potentially promising new therapeutic candidate for the treatment of ischemic stroke.","twitter:creator":"@lifetein","twitter:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg"},"aioseo_meta_data":{"post_id":"468","title":"Trans-activator of transcription (Tat) with nuclear translocation signal peptide drug candidate | #site_title","description":"The Tat sequence is originally from a crucial non-structural protein of human immunodeficiency virus (HIV). This 11-amino acid positively charged TAT peptide can pull diverse molecules across cell membranes in vitro and in vivo. Trans-activator of transcription (Tat) is a cell-penetrating peptide. The peptide can translocate numerous proteins, peptides, DNA, RNA, and small drugs into the cytoplasm with high efficiency. The Tat-NTS (NH2-YGRKKRRQRRR-RSFPHLRRVF-CONH2) peptide was found to specifically block the interaction of ANXA1 with importin \u03b2. This blocking would inhibit the nuclear translocation of ANXA1, consequently protecting neurons from ischemic stroke damage. So the Tat-NTS peptide can be used as a novel and potentially promising new therapeutic candidate for the treatment of ischemic stroke.","keywords":[{"label":"tat","value":"tat"},{"label":"CPP","value":"CPP"},{"label":"Trans-activator of transcription","value":"Trans-activator of transcription"},{"label":"nuclear translocation signal","value":"nuclear translocation signal"},{"label":"peptide synthesis","value":"peptide synthesis"}],"keyphrases":{"focus":{"keyphrase":"Nuclear Translocation Signal Peptide","score":54,"analysis":{"keyphraseInTitle":{"score":9,"maxScore":9,"error":0},"keyphraseInDescription":{"score":3,"maxScore":9,"error":1},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":4},"keyphraseInURL":{"score":5,"maxScore":5,"error":0},"keyphraseInIntroduction":{"score":3,"maxScore":9,"error":1},"keyphraseInSubHeadings":[],"keyphraseInImageAlt":{"score":3,"maxScore":9,"error":1},"keywordDensity":{"score":0,"type":"low","maxScore":9,"error":1}}},"additional":[{"keyphrase":"tat peptide","score":80,"analysis":{"keyphraseInDescription":{"score":9,"maxScore":9,"error":0},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":2},"keyphraseInIntroduction":{"score":9,"maxScore":9,"error":0},"keyphraseInImageAlt":{"score":9,"maxScore":9,"error":0},"keywordDensity":{"score":0,"type":"low","maxScore":9,"error":1}}}]},"primary_term":null,"canonical_url":null,"og_title":null,"og_description":null,"og_object_type":"article","og_image_type":"default","og_image_url":null,"og_image_width":null,"og_image_height":null,"og_image_custom_url":null,"og_image_custom_fields":null,"og_video":"","og_custom_url":null,"og_article_section":null,"og_article_tags":null,"twitter_use_og":true,"twitter_card":"summary","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":[],"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"Article","isEnabled":true},"graphs":[]},"schema_type":null,"schema_type_options":null,"pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":"-1","robots_max_videopreview":"-1","robots_max_imagepreview":"none","priority":null,"frequency":"default","location":null,"local_seo":null,"seo_analyzer_scan_date":null,"breadcrumb_settings":null,"limit_modified_date":false,"open_ai":"{\"title\":{\"suggestions\":[],\"usage\":0},\"description\":{\"suggestions\":[],\"usage\":0}}","ai":null,"created":"2020-12-04 22:27:48","updated":"2025-06-18 02:47:18"},"aioseo_breadcrumb":"

\n\t\t\tHome<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tPeptide<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tNew Drug Candidate: Tat Protein with Nuclear Translocation Signal Peptide\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/www.lifetein.com\/blog"},{"label":"Peptide","link":"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/"},{"label":"New Drug Candidate: Tat Protein with Nuclear Translocation Signal Peptide","link":"https:\/\/www.lifetein.com\/blog\/trans-activator-of-transcription-tat-with-nuclear-translocation-signal-peptide-drug-candidate\/"}],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/468","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=468"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/468\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/487"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=468"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=468"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=468"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}