![\"Antibody-Conjugate\"](\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/03\/Antibody-Conjugate-300x73.png\")
Antibody-Conjugate<\/p><\/div>\n
<\/h2>\nPreparing Antibody-Peptide, Antibody Oligonucleotide or Antibody-Compound Conjugates<\/h2>\n
1. Conjugation of DBCO to the Antibody. The DBCO-PEG5-NHS was used to react with the NH2 groups on the antibody. The inclusion of a PEG5 linker improves the water solubility of the hydrophobic DBCO, introduces a spacer, and increases flexibility between the antibody molecule and the peptide\/oligonucleotide or compounds. This will alleviate the antibody’s steric effect on the enzymatic reactions.<\/p>\n
2. Prepare the azido-peptide or azido-oligonucleotide. LifeTein provides click chemistry modified peptide synthesis: N-terminal azide-peptide\/oligo or C-terminal peptide\/oligo-azide.<\/p>\n
3. Covalent attachment of the peptide\/oligonucleotide to the antibody. The reaction between DBCO and azide is slow compared to the CuAAC reaction. The 16\u201318 h reaction time in PBS at 4 \u00b0C is ideal for increasing the final product yield. The DBCO-antibody in the intermediate reaction is stable.<\/p>\n
https:\/\/pubs.acs.org\/doi\/full\/10.1021\/acs.bioconjchem.5b00613<\/p>\n","protected":false},"excerpt":{"rendered":"
We describe a simple method for preparing antibody-peptide, antibody-oligonucleotide, or antibody-compound conjugates and discuss its applications in drug delivery and new drug design. Conjugation is based on alkyne-azide cycloaddition. This Cu-free click reaction starts from the dibenzocyclooctyne (DBCO) moiety-activated antibodies … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":450,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[102,99,105,107,106,108,104,100,103],"class_list":["post-449","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-antibody-conjugate","tag-click-chemistry","tag-click-chemistry-fluorescence-labeling","tag-click-chemistry-in-peptide-based-drug-design","tag-click-chemistry-modified-peptide-synthesis","tag-copper-catalyzed-azide-alkyne-click-chemistry-for-bioconjugation","tag-dbco-antibody-conjugation","tag-dbco-azide","tag-peptide-azide"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"Simple method to prepare antibody-peptide\/oligonucleotide conjugate | LifeTein Peptide Blog","description":"We describe a simple method for preparing antibody-peptide, antibody-oligonucleotide or antibody-compound conjugates and discuss its applications in drug delivery and new drug design. 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