{"id":444,"date":"2018-03-20T16:26:20","date_gmt":"2018-03-20T20:26:20","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=444"},"modified":"2024-11-16T22:10:53","modified_gmt":"2024-11-17T03:10:53","slug":"a-simple-protocol-maleimide-labeling-of-peptide-and-other-thiolated-biomolecules","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/a-simple-protocol-maleimide-labeling-of-peptide-and-other-thiolated-biomolecules\/","title":{"rendered":"A simple protocol: Maleimide labeling of peptide and other thiolated biomolecules"},"content":{"rendered":"\n
\"Cy5.5
Cy5.5 peptide<\/figcaption><\/figure>\n\n\n

The reaction of maleimides with thiols is widely used for bioconjugation and labeling biomolecules such as proteins and peptides. Maleimides are electrophilic compounds that show high selectivity towards thiols.<\/p>\n

The Reaction of Maleimides With Thiols<\/h2>\n

1. Dissolve the peptide or other biomolecules containing thiol in a degassed buffer (PBS, Tris, or HEPES) at pH 7-7.5.
2. Add a 100x molar excess of TCEP (tris-carboxyethyl phosphine) reagent to reduce disulfide bonds.
3. Dissolve maleimide in DMSO or fresh DMF (1-10mg in 100uL).
4. Add dye solution such as cy5 maleimide to thiol solution (20x fold excess of dye), flush\u00a0with an inert gas, and close tightly.
5. Mix thoroughly and keep at room temperature or 4C overnight.
6. Purify by gel filtration, HPLC, FPLC, or electrophoresis.<\/p>\n

\u00a0<\/p>\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

The reaction of maleimides with thiols is widely used for bioconjugation and labeling biomolecules such as proteins and peptides. Maleimides are electrophilic compounds that show high selectivity towards thiols. The Reaction of Maleimides With Thiols 1. Dissolve the peptide or … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":1900,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[97,98],"class_list":["post-444","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-maleimide","tag-peptide-conjugation"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"Maleimide labeling of peptide and other thiolated biomolecules | LifeTein Peptide Blog","description":"The reaction of maleimides with thiols is widely used for bioconjugation and labeling of biomolecules such as proteins and peptides. Maleimides are electrophilic compounds which show high selectivity towards thiols.","canonical_url":"https:\/\/www.lifetein.com\/blog\/a-simple-protocol-maleimide-labeling-of-peptide-and-other-thiolated-biomolecules\/","robots":"max-image-preview:large","keywords":"maleimide thiol click chemistry,peptide conjugation,maleimide","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"article","og:title":"Maleimide labeling of peptide and other thiolated biomolecules | LifeTein Peptide Blog","og:description":"The reaction of maleimides with thiols is widely used for bioconjugation and labeling of biomolecules such as proteins and peptides. Maleimides are electrophilic compounds which show high selectivity towards thiols.","og:url":"https:\/\/www.lifetein.com\/blog\/a-simple-protocol-maleimide-labeling-of-peptide-and-other-thiolated-biomolecules\/","fb:admins":"233983946638783","og:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","og:image:secure_url":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","article:tag":["maleimide thiol click chemistry","peptide conjugation","maleimide","peptide"],"article:published_time":"2018-03-20T20:26:20+00:00","article:modified_time":"2024-11-17T03:10:53+00:00","article:publisher":"https:\/\/www.facebook.com\/LifeTein-LLC-233983946638783\/","twitter:card":"summary","twitter:site":"@lifetein","twitter:title":"Maleimide labeling of peptide and other thiolated biomolecules | LifeTein Peptide Blog","twitter:description":"The reaction of maleimides with thiols is widely used for bioconjugation and labeling of biomolecules such as proteins and peptides. Maleimides are electrophilic compounds which show high selectivity towards thiols.","twitter:creator":"@lifetein","twitter:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg"},"aioseo_meta_data":{"post_id":"444","title":"Maleimide labeling of peptide and other thiolated biomolecules | #site_title","description":"The reaction of maleimides with thiols is widely used for bioconjugation and labeling of biomolecules such as proteins and peptides. Maleimides are electrophilic compounds which show high selectivity towards thiols.","keywords":[{"label":"maleimide thiol click chemistry","value":"maleimide thiol click chemistry"},{"label":"peptide conjugation","value":"peptide conjugation"}],"keyphrases":{"focus":{"keyphrase":"","score":0,"analysis":{"keyphraseInTitle":{"score":0,"maxScore":9,"error":1}}},"additional":[]},"primary_term":null,"canonical_url":null,"og_title":null,"og_description":null,"og_object_type":"article","og_image_type":"default","og_image_url":null,"og_image_width":null,"og_image_height":null,"og_image_custom_url":null,"og_image_custom_fields":null,"og_video":"","og_custom_url":null,"og_article_section":null,"og_article_tags":null,"twitter_use_og":true,"twitter_card":"summary","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":{"id":"aioseo-article-633d750124b93","slug":"article","graphName":"Article","label":"Article","properties":{"type":"BlogPosting","name":"#post_title","headline":"#post_title","description":"#post_excerpt","image":"","keywords":"","author":{"name":"#author_name","url":"#author_url"},"dates":{"include":true,"datePublished":"","dateModified":""}}},"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"Article","isEnabled":true},"graphs":[]},"schema_type":"default","schema_type_options":"{\"article\":{\"articleType\":\"BlogPosting\"},\"course\":{\"name\":\"\",\"description\":\"\",\"provider\":\"\"},\"faq\":{\"pages\":[]},\"product\":{\"reviews\":[]},\"recipe\":{\"ingredients\":[],\"instructions\":[],\"keywords\":[]},\"software\":{\"reviews\":[],\"operatingSystems\":[]},\"webPage\":{\"webPageType\":\"WebPage\"}}","pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":"-1","robots_max_videopreview":"-1","robots_max_imagepreview":"none","priority":null,"frequency":"default","location":null,"local_seo":null,"seo_analyzer_scan_date":null,"breadcrumb_settings":null,"limit_modified_date":false,"open_ai":"{\"title\":{\"suggestions\":[],\"usage\":0},\"description\":{\"suggestions\":[],\"usage\":0}}","ai":null,"created":"2020-12-04 22:27:49","updated":"2025-06-18 02:45:17"},"aioseo_breadcrumb":"

\n\t\t\tHome<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tPeptide<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tA simple protocol: Maleimide labeling of peptide and other thiolated biomolecules\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/www.lifetein.com\/blog"},{"label":"Peptide","link":"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/"},{"label":"A simple protocol: Maleimide labeling of peptide and other thiolated biomolecules","link":"https:\/\/www.lifetein.com\/blog\/a-simple-protocol-maleimide-labeling-of-peptide-and-other-thiolated-biomolecules\/"}],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/444","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=444"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/444\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/1900"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=444"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=444"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=444"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}