{"id":435,"date":"2018-02-27T15:43:23","date_gmt":"2018-02-27T20:43:23","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=435"},"modified":"2024-11-16T22:16:18","modified_gmt":"2024-11-17T03:16:18","slug":"long-peptide-synthesis-by-click-chemistry","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/long-peptide-synthesis-by-click-chemistry\/","title":{"rendered":"Long peptide synthesis by click chemistry"},"content":{"rendered":"<p>Some fusion or chimeric proteins could never be produced from the E.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. Obtaining peptides sized 100\u2013200 amino acids using chemical synthesis is much faster and cheaper than cloning and overexpressing in Escherichia coli. In addition, the resulting peptide is always correct. Chemical synthesis can regularly incorporate non-genetically encoded structures, such as D-amino acids, into the protein. Synthetic peptides eliminate problems such as poor or no expression, cloning errors, tags like FLAG or 6-His, or the mistranslation of non-preferred codons in prokaryotic hosts. Artificial amino acids that have isosteric side chains can be used to investigate the functional importance of specific residues. The peptide design and synthesis using click chemistry can achieve all these chimeric proteins.<\/p>\n<div id=\"attachment_436\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" aria-describedby=\"caption-attachment-436\" class=\"size-medium wp-image-436\" src=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/Long-peptide-synthesis-by-ligation-300x250.png\" alt=\"Long peptide synthesis by click chemistry\" width=\"300\" height=\"250\" srcset=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/Long-peptide-synthesis-by-ligation-300x250.png 300w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/Long-peptide-synthesis-by-ligation-768x640.png 768w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/Long-peptide-synthesis-by-ligation-1024x853.png 1024w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/Long-peptide-synthesis-by-ligation-360x300.png 360w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-436\" class=\"wp-caption-text\">Long peptide synthesis by click chemistry<\/p><\/div>\n<p>\u00a0<\/p>\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Some fusion or chimeric proteins could never be produced from the E.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. Obtaining peptides sized 100\u2013200 amino acids using chemical synthesis is much faster and cheaper than &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/long-peptide-synthesis-by-click-chemistry\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":2093,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[90,91,92,93],"class_list":["post-435","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-chimeric-protein","tag-fusion-protein","tag-long-peptide-synthesis","tag-long-protein-synthesis"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 4.9.9 - aioseo.com -->\n\t<meta name=\"description\" content=\"The chimeric proteins can be achieved by the peptide design and synthesis using the click chemistry. Some fusion protein or chimeric proteins could never be produced from the e.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. 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Some fusion protein or chimeric proteins could never be produced from the e.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. Obtaining peptides sized 100\u2013200 amino acids using chemical synthesis is much faster and cheaper than cloning and overexpressing in Escherichia coli.\" \/>\n\t\t<meta name=\"twitter:creator\" content=\"@lifetein\" \/>\n\t\t<meta name=\"twitter:image\" content=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg\" \/>\n\t\t<!-- All in One SEO -->\n\n","aioseo_head_json":{"title":"Long peptide synthesis by click chemistry | LifeTein Peptide Blog","description":"The chimeric proteins can be achieved by the peptide design and synthesis using the click chemistry. Some fusion protein or chimeric proteins could never be produced from the e.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. Obtaining peptides sized 100\u2013200 amino acids using chemical synthesis is much faster and cheaper than cloning and overexpressing in Escherichia coli.","canonical_url":"https:\/\/www.lifetein.com\/blog\/long-peptide-synthesis-by-click-chemistry\/","robots":"max-image-preview:large","keywords":"long peptide synthesis,chimeric proteins,fusion protein,chimeric protein,long protein synthesis","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"article","og:title":"Long peptide synthesis by click chemistry | LifeTein Peptide Blog","og:description":"The chimeric proteins can be achieved by the peptide design and synthesis using the click chemistry. 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Some fusion protein or chimeric proteins could never be produced from the e.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. Obtaining peptides sized 100\u2013200 amino acids using chemical synthesis is much faster and cheaper than cloning and overexpressing in Escherichia coli.","twitter:creator":"@lifetein","twitter:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg"},"aioseo_meta_data":{"post_id":"435","title":"Long peptide synthesis by click chemistry | #site_title","description":"The chimeric proteins can be achieved by the peptide design and synthesis using the click chemistry. Some fusion protein or chimeric proteins could never be produced from the e.coli expression system, especially when several hydrophobic sequences are involved in the functional domains. 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