{"id":426,"date":"2018-02-15T15:56:07","date_gmt":"2018-02-15T20:56:07","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=426"},"modified":"2024-11-16T22:18:45","modified_gmt":"2024-11-17T03:18:45","slug":"noble-metal-gold-and-silver-nanoparticle-are-conjugated-with-peptides-for-cellular-imaging","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/noble-metal-gold-and-silver-nanoparticle-are-conjugated-with-peptides-for-cellular-imaging\/","title":{"rendered":"Noble metal gold and silver nanoparticle are conjugated with peptides for cellular imaging"},"content":{"rendered":"<p>Noble metal gold (Au) and silver (Ag) nanoparticles (NPs) are used to conjugate with M3 peptides. The AuNPs-sGFP and AuNPs-M3 peptide forms an active SERS hot spot through self-assembly and GFP complementation. The nanoparticles self-assemble into surface-enhanced Raman-scattering (SERS) nanoclusters. The nanocluster can be a contrast agent for multimodal SERS and photoacoustic microscopy with single-cell sensitivity.<\/p>\n<div id=\"attachment_427\" style=\"width: 310px\" class=\"wp-caption aligncenter\"><img decoding=\"async\" aria-describedby=\"caption-attachment-427\" class=\"size-medium wp-image-427\" src=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/AuNPs-coated-with-M3-peptides-GFP-300x142.png\" alt=\"AuNPs coated with M3 peptides-GFP\" width=\"300\" height=\"142\" srcset=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/AuNPs-coated-with-M3-peptides-GFP-300x142.png 300w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/AuNPs-coated-with-M3-peptides-GFP-768x365.png 768w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/AuNPs-coated-with-M3-peptides-GFP-1024x486.png 1024w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/AuNPs-coated-with-M3-peptides-GFP-500x237.png 500w, https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2018\/02\/AuNPs-coated-with-M3-peptides-GFP.png 1866w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><p id=\"caption-attachment-427\" class=\"wp-caption-text\">AuNPs coated with M3 peptides-GFP<\/p><\/div>\n<p>Reference: M3 peptide was purchased from LifeTein.<\/p>\n<h2 class=\"tighten-line-height small-space-below\" data-test=\"article-title\">Cellular imaging by targeted assembly of hot-spot SERS and photoacoustic nanoprobes using split-fluorescent protein scaffolds<\/h2>\n<p>https:\/\/www.nature.com\/articles\/s41467-018-03046-w<\/p>\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Noble metal gold (Au) and silver (Ag) nanoparticles (NPs) are used to conjugate with M3 peptides. The AuNPs-sGFP and AuNPs-M3 peptide forms an active SERS hot spot through self-assembly and GFP complementation. The nanoparticles self-assemble into surface-enhanced Raman-scattering (SERS) nanoclusters. &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/noble-metal-gold-and-silver-nanoparticle-are-conjugated-with-peptides-for-cellular-imaging\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":1383,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_crdt_document":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[83,85,84],"class_list":["post-426","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-gfp","tag-hot-spot","tag-m3-peptide"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/426","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=426"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/426\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/1383"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=426"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=426"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=426"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}