{"id":294,"date":"2016-01-14T10:42:26","date_gmt":"2016-01-14T15:42:26","guid":{"rendered":"http:\/\/www.lifetein.com\/blog\/?p=294"},"modified":"2024-11-16T22:30:32","modified_gmt":"2024-11-17T03:30:32","slug":"look-whos-talking","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/look-whos-talking\/","title":{"rendered":"Look Who\u2019s Talking"},"content":{"rendered":"<p>Intraspecies pheromone signaling regulated by proteases is critical for fungi procreation. \u00a0The fungal aspartyl protease Bar1 was shown to have unique substrate specificity of important implications in fungal evolution.\u00a0 LifeTein synthesized substrate peptides of Bar1, dual-tagged with DABCYL and EDANS, from the sequence of \u03b1 pheromone, the native target of Bar1. Referred to as internally quenched or IQ peptides, they were used in fluorescence resonance energy transfer (FRET) assays to study the enzyme kinetics of Bar1.<\/p>\n<h2 style=\"text-align: center;\">LifeTein&#8217;s Internally Quenched Peptides<\/h2>\n<p><strong>mBio<\/strong> 6(6):e01604-15. doi:10.1128\/mBio.01604-15. <a href=\"http:\/\/mbio.asm.org\/content\/6\/6\/e01604-15.full\">Evolutionary selection on barrier activity: Bar1 is an aspartyl protease with novel substrate specificity.<\/a><\/p>\n\n\n<p class=\"has-text-align-center wp-block-paragraph\"><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Intraspecies pheromone signaling regulated by proteases is critical for fungi procreation. \u00a0The fungal aspartyl protease Bar1 was shown to have unique substrate specificity of important implications in fungal evolution.\u00a0 LifeTein synthesized substrate peptides of Bar1, dual-tagged with DABCYL and EDANS, &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/look-whos-talking\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":1853,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[50,14],"class_list":["post-294","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-biotin-peptide","tag-cancer-peptide"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 4.9.10 - aioseo.com -->\n\t<meta name=\"description\" content=\"The fungal aspartyl protease Bar1 was shown to have unique substrate specificity of important implications in fungal evolution. 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22:27:49","updated":"2025-06-18 02:43:16"},"aioseo_breadcrumb":"<div class=\"aioseo-breadcrumbs\"><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/www.lifetein.com\/blog\" title=\"Home\">Home<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">&raquo;<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/\" title=\"Peptide\">Peptide<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">&raquo;<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\tLook Who\u2019s Talking\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/www.lifetein.com\/blog"},{"label":"Peptide","link":"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/"},{"label":"Look Who\u2019s 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