{"id":284,"date":"2016-01-14T10:39:57","date_gmt":"2016-01-14T15:39:57","guid":{"rendered":"http:\/\/www.lifetein.com\/blog\/?p=284"},"modified":"2024-11-16T22:37:14","modified_gmt":"2024-11-17T03:37:14","slug":"to-make-simpler-and-better-biosensors","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/to-make-simpler-and-better-biosensors\/","title":{"rendered":"To Make Simpler and Better Biosensors"},"content":{"rendered":"<p>To improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which Trp residues of scFv internally quench their fluorescence.\u00a0 Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.<\/p>\n<h2 style=\"text-align: center;\">Making Simpler &amp; Better Biosensors<\/h2>\n<p><strong>ACS Sensors<\/strong>, Article ASAP. DOI<strong>: <\/strong>10.1021\/acssensors.5b00089.\u00a0 <a href=\"http:\/\/pubs.acs.org\/doi\/full\/10.1021\/acssensors.5b00089\">Q-Bodies from Recombinant Single-Chain Fv Fragment with Better Yield and Expanded Palette of Fluorophores.<\/a><\/p>\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>To improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which Trp residues of scFv internally quench their fluorescence.\u00a0 Unlabeled and biotinylated BGP &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/to-make-simpler-and-better-biosensors\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":2041,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[4],"tags":[64,65],"class_list":["post-284","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-biotinylated-peptide","tag-fluorescent-labeled-peptide"],"aioseo_notices":[],"aioseo_head":"\n\t\t<!-- All in One SEO 4.9.8 - aioseo.com -->\n\t<meta name=\"description\" content=\"In order to improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which their fluorescence is internally quenched by Trp residues of scFv. Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.\" \/>\n\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t<meta name=\"author\" content=\"LifeTein Peptide\"\/>\n\t<meta name=\"google-site-verification\" content=\"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc\" \/>\n\t<meta name=\"msvalidate.01\" content=\"5B15601215D876311F84CFEFB0F72F18\" \/>\n\t<meta name=\"keywords\" content=\"fluorescent labeled peptide,peptide synthesis,biotinylated peptide\" \/>\n\t<link rel=\"canonical\" href=\"https:\/\/www.lifetein.com\/blog\/to-make-simpler-and-better-biosensors\/\" \/>\n\t<meta name=\"generator\" content=\"All in One SEO (AIOSEO) 4.9.8\" \/>\n\t\t<meta property=\"og:locale\" content=\"en_US\" \/>\n\t\t<meta property=\"og:site_name\" content=\"LifeTein Peptide Synthesis Blog\" \/>\n\t\t<meta property=\"og:type\" content=\"activity\" \/>\n\t\t<meta property=\"og:title\" content=\"To Make Simpler and Better Biosensors | LifeTein Peptide Blog\" \/>\n\t\t<meta property=\"og:description\" content=\"In order to improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which their fluorescence is internally quenched by Trp residues of scFv. 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Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.\" \/>\n\t\t<meta name=\"twitter:creator\" content=\"@lifetein\" \/>\n\t\t<meta name=\"twitter:image\" content=\"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg\" \/>\n\t\t<!-- All in One SEO -->\n\n","aioseo_head_json":{"title":"To Make Simpler and Better Biosensors | LifeTein Peptide Blog","description":"In order to improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which their fluorescence is internally quenched by Trp residues of scFv. Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.","canonical_url":"https:\/\/www.lifetein.com\/blog\/to-make-simpler-and-better-biosensors\/","robots":"max-image-preview:large","keywords":"fluorescent labeled peptide,peptide synthesis,biotinylated peptide","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"activity","og:title":"To Make Simpler and Better Biosensors | LifeTein Peptide Blog","og:description":"In order to improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which their fluorescence is internally quenched by Trp residues of scFv. Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.","og:url":"https:\/\/www.lifetein.com\/blog\/to-make-simpler-and-better-biosensors\/","fb:admins":"233983946638783","og:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","og:image:secure_url":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","twitter:card":"summary","twitter:site":"@lifetein","twitter:title":"To Make Simpler and Better Biosensors | LifeTein Peptide Blog","twitter:description":"In order to improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which their fluorescence is internally quenched by Trp residues of scFv. Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.","twitter:creator":"@lifetein","twitter:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg"},"aioseo_meta_data":{"post_id":"284","title":"To Make Simpler and Better Biosensors | #site_title","description":"In order to improve the design of immunosensors, fluorophores were conjugated on to the single chain Fv (scFv) fragment of a recombinant anti-BGP antibody to form Quenchbodies (Q-bodies), in which their fluorescence is internally quenched by Trp residues of scFv.\u00a0 Unlabeled and biotinylated BGP peptides made by LifeTein were used in ELISA and FRET assays as the ligand to evaluate the quality of Qbodies, and to demonstrate the antigen-dependent fluorescence of Qbodies.","keywords":[{"label":"fluorescent labeled 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22:27:49","updated":"2025-06-18 02:41:15"},"aioseo_breadcrumb":"<div class=\"aioseo-breadcrumbs\"><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/www.lifetein.com\/blog\" title=\"Home\">Home<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">&raquo;<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\t<a href=\"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/\" title=\"Peptide\">Peptide<\/a>\n\t\t<\/span><span class=\"aioseo-breadcrumb-separator\">&raquo;<\/span><span class=\"aioseo-breadcrumb\">\n\t\t\tTo Make Simpler and Better Biosensors\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/www.lifetein.com\/blog"},{"label":"Peptide","link":"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/"},{"label":"To Make Simpler and Better 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