{"id":1247,"date":"2022-07-27T16:00:11","date_gmt":"2022-07-27T20:00:11","guid":{"rendered":"https:\/\/www.lifetein.com\/blog\/?p=1247"},"modified":"2024-11-16T21:20:08","modified_gmt":"2024-11-17T02:20:08","slug":"lpetg-peptide-and-srta-reaction-allows-analysis-of-cell-cell-interactions-both-in-vitro-and-in-vivo","status":"publish","type":"post","link":"https:\/\/www.lifetein.com\/blog\/lpetg-peptide-and-srta-reaction-allows-analysis-of-cell-cell-interactions-both-in-vitro-and-in-vivo\/","title":{"rendered":"Revolutionary LIPSTIC Method with LPETG Peptide Illuminates Receptor-Ligand Interactions In Vivo And In Vitro"},"content":{"rendered":"\n
\"LPETG
-LIPTSTIC mechanism, from the cited paper.<\/figcaption><\/figure>\n\n\n\n

Cell interaction analysis is a cornerstone of biological research, providing critical insights into the intricate world of molecular communication within living organisms. While traditional microscopy offers a glimpse into these interactions, it often falls short when it comes to revealing the specific receptors and ligands involved. Enter a groundbreaking method known as Labeling Immune Partnerships by SorTagging Intercellular Contacts, or LIPSTIC for short, which has been developed by a team of innovative scientists using LPETG Peptide.<\/p>\n\n\n\n

At the heart of LIPSTIC lies the ingenious combination of a fluorescent LPXTG peptide motif and Staphylococcus aureus transpeptidase Sortase A (SrtA), offering a highly effective means of tracking and studying cell interactions. This novel approach is readily detectable through flow cytometry, making it a game-changer in the field of biological research.<\/p>\n\n\n\n

The LIPSTIC method hinges on the LPETG peptide and SrtA reaction, a technique that allows for the labeling of receptor and ligand interactions. LifeTein, a leading supplier in the life sciences industry, played a pivotal role by providing the necessary Biotin-ahx-LPETG<\/a> peptide to the research group. In the LIPSTIC method, a noteworthy ligand or receptor is fused with a tag composed of five N-terminal glycine residues (G5). The SrtA enzyme then graciously donates the fluorescent peptide to this fusion, enabling precise monitoring of the acceptor cell post-separation.<\/p>\n\n\n\n

One of the most impressive aspects of LIPSTIC is its versatility. It empowers scientists to analyze cell-cell interactions both in vitro and in vivo, offering a comprehensive understanding of molecular partnerships in various biological contexts. Moreover, LIPSTIC’s sensitivity is a standout feature, as it can even detect rare or low-intensity interactions that might have otherwise remained hidden.<\/p>\n\n\n\n

In conclusion, the introduction of the LIPSTIC method marks a significant advancement in the field of cell interaction analysis. Its ability to unveil the intricacies of receptor-ligand interactions in living systems, along with its applicability in diverse research settings, positions LIPSTIC as a powerful tool for scientists striving to unlock the secrets of cellular communication.<\/p>\n\n\n\n


Pasqual G, Chudnovskiy A, Tas JMJ, Agudelo M, Schweitzer LD, Cui A, Hacohen N, Victora GD. Monitoring T cell-dendritic cell interactions in vivo by intercellular enzymatic labeling. Nature. 2018 Jan 25;553(7689):496-500. doi: 10.1038\/nature25442. Epub 2018 Jan 17. PMID: 29342141; PMCID: PMC5853129.<\/p>\n","protected":false},"excerpt":{"rendered":"

Cell interaction analysis is a cornerstone of biological research, providing critical insights into the intricate world of molecular communication within living organisms. While traditional microscopy offers a glimpse into these interactions, it often falls short when it comes to revealing … Continue reading →<\/span><\/a><\/p>\n","protected":false},"author":6,"featured_media":1526,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"footnotes":""},"categories":[4],"tags":[165,166,167,168,171,170,173,161,164,163,27,172,162,169],"class_list":["post-1247","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-peptide_synthesis","tag-biotin-lpetg","tag-cell","tag-cell-cell","tag-interaction","tag-labeling","tag-lifetein","tag-ligand","tag-lpetg","tag-lpetgs","tag-lpxtg","tag-peptide","tag-receptor","tag-srta","tag-staphylococcus-aureus"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\n","aioseo_head_json":{"title":"Revolutionary LIPSTIC Method with LPETG Peptide Illuminates Receptor-Ligand Interactions In Vivo And In Vitro | LifeTein Peptide Blog","description":"LPETG Peptide and SrtA Reaction allows Analysis of cell-cell Interactions both in vitro and in vivo with LPETG Peptide","canonical_url":"https:\/\/www.lifetein.com\/blog\/lpetg-peptide-and-srta-reaction-allows-analysis-of-cell-cell-interactions-both-in-vitro-and-in-vivo\/","robots":"max-image-preview:large","keywords":"biotin-lpetg,cell,cell-cell,interaction,labeling,lifetein,ligand,lpetg,lpetgs,lpxtg,peptide,receptor,srta,staphylococcus aureus","webmasterTools":{"google-site-verification":"xKDW6DUiGuZ6nwglNeWlVWTgzTtMPzas13ROL63ytTc","msvalidate.01":"5B15601215D876311F84CFEFB0F72F18","miscellaneous":""},"schema":null,"og:locale":"en_US","og:site_name":"LifeTein Peptide Synthesis Blog","og:type":"article","og:title":"Revolutionary LIPSTIC Method with LPETG Peptide Illuminates Receptor-Ligand Interactions In Vivo And In Vitro | LifeTein Peptide Blog","og:description":"LPETG Peptide and SrtA Reaction allows Analysis of cell-cell Interactions both in vitro and in vivo with LPETG Peptide","og:url":"https:\/\/www.lifetein.com\/blog\/lpetg-peptide-and-srta-reaction-allows-analysis-of-cell-cell-interactions-both-in-vitro-and-in-vivo\/","fb:admins":"233983946638783","og:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","og:image:secure_url":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg","article:tag":["biotin-lpetg","cell","cell-cell","interaction","labeling","lifetein","ligand","lpetg","lpetgs","lpxtg","peptide","receptor","srta","staphylococcus aureus"],"article:published_time":"2022-07-27T20:00:11+00:00","article:modified_time":"2024-11-17T02:20:08+00:00","article:publisher":"https:\/\/www.facebook.com\/LifeTein-LLC-233983946638783\/","twitter:card":"summary_large_image","twitter:site":"@lifetein","twitter:title":"Revolutionary LIPSTIC Method with LPETG Peptide Illuminates Receptor-Ligand Interactions In Vivo And In Vitro | LifeTein Peptide Blog","twitter:description":"LPETG Peptide and SrtA Reaction allows Analysis of cell-cell Interactions both in vitro and in vivo with LPETG Peptide","twitter:creator":"@lifetein","twitter:image":"https:\/\/www.lifetein.com\/blog\/wp-content\/uploads\/2020\/02\/LifeTein-Blog1-2-scaled.jpg"},"aioseo_meta_data":{"post_id":"1247","title":null,"description":"LPETG Peptide and SrtA Reaction allows Analysis of cell-cell Interactions both in vitro and in vivo with LPETG Peptide","keywords":null,"keyphrases":{"focus":{"keyphrase":"LPETG Peptide","score":100,"analysis":{"keyphraseInTitle":{"score":9,"maxScore":9,"error":0},"keyphraseInDescription":{"score":9,"maxScore":9,"error":0},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":2},"keyphraseInURL":{"score":5,"maxScore":5,"error":0},"keyphraseInIntroduction":{"score":9,"maxScore":9,"error":0},"keyphraseInSubHeadings":[],"keyphraseInImageAlt":{"score":9,"maxScore":9,"error":0},"keywordDensity":{"type":"best","score":9,"maxScore":9,"error":0}}},"additional":[{"keyphrase":"LPETG Peptide","score":100,"analysis":{"keyphraseInDescription":{"score":9,"maxScore":9,"error":0},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":2},"keyphraseInIntroduction":{"score":9,"maxScore":9,"error":0},"keyphraseInImageAlt":{"score":9,"maxScore":9,"error":0},"keywordDensity":{"type":"best","score":9,"maxScore":9,"error":0}}},{"keyphrase":"Biotin-ahx-LPETG","score":40,"analysis":{"keyphraseInDescription":{"score":3,"maxScore":9,"error":1},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":1},"keyphraseInIntroduction":{"score":3,"maxScore":9,"error":1},"keyphraseInImageAlt":{"score":3,"maxScore":9,"error":1},"keywordDensity":{"score":0,"type":"low","maxScore":9,"error":1}}},{"keyphrase":"LIPSTIC Method","score":60,"analysis":{"keyphraseInDescription":{"score":3,"maxScore":9,"error":1},"keyphraseLength":{"score":9,"maxScore":9,"error":0,"length":2},"keyphraseInIntroduction":{"score":3,"maxScore":9,"error":1},"keyphraseInImageAlt":{"score":3,"maxScore":9,"error":1},"keywordDensity":{"type":"best","score":9,"maxScore":9,"error":0}}}]},"primary_term":null,"canonical_url":null,"og_title":null,"og_description":null,"og_object_type":"default","og_image_type":"default","og_image_url":null,"og_image_width":null,"og_image_height":null,"og_image_custom_url":null,"og_image_custom_fields":null,"og_video":"","og_custom_url":null,"og_article_section":null,"og_article_tags":null,"twitter_use_og":false,"twitter_card":"default","twitter_image_type":"default","twitter_image_url":null,"twitter_image_custom_url":null,"twitter_image_custom_fields":null,"twitter_title":null,"twitter_description":null,"schema":{"blockGraphs":[],"customGraphs":[],"default":{"data":{"Article":{"id":"aioseo-article-633cfb6d7182c","slug":"article","graphName":"Article","label":"Article","properties":{"type":"BlogPosting","name":"#post_title","headline":"#post_title","description":"#post_excerpt","image":"","keywords":"","author":{"name":"#author_name","url":"#author_url"},"dates":{"include":true,"datePublished":"","dateModified":""}}},"Course":[],"Dataset":[],"FAQPage":[],"Movie":[],"Person":[],"Product":[],"ProductReview":[],"Car":[],"Recipe":[],"Service":[],"SoftwareApplication":[],"WebPage":[]},"graphName":"Article","isEnabled":true},"graphs":[]},"schema_type":"default","schema_type_options":"{\"article\":{\"articleType\":\"BlogPosting\"},\"course\":{\"name\":\"\",\"description\":\"\",\"provider\":\"\"},\"faq\":{\"pages\":[]},\"product\":{\"reviews\":[]},\"recipe\":{\"ingredients\":[],\"instructions\":[],\"keywords\":[]},\"software\":{\"reviews\":[],\"operatingSystems\":[]},\"webPage\":{\"webPageType\":\"WebPage\"},\"blockGraphs\":[]}","pillar_content":false,"robots_default":true,"robots_noindex":false,"robots_noarchive":false,"robots_nosnippet":false,"robots_nofollow":false,"robots_noimageindex":false,"robots_noodp":false,"robots_notranslate":false,"robots_max_snippet":"-1","robots_max_videopreview":"-1","robots_max_imagepreview":"large","priority":null,"frequency":"default","location":null,"local_seo":null,"seo_analyzer_scan_date":null,"breadcrumb_settings":null,"limit_modified_date":false,"open_ai":"{\"title\":{\"suggestions\":[],\"usage\":0},\"description\":{\"suggestions\":[],\"usage\":0}}","ai":null,"created":"2022-07-21 13:50:44","updated":"2025-06-18 03:01:25"},"aioseo_breadcrumb":"

\n\t\t\tHome<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tPeptide<\/a>\n\t\t<\/span>»<\/span>\n\t\t\tRevolutionary LIPSTIC Method with LPETG Peptide Illuminates Receptor-Ligand Interactions In Vivo And In Vitro\n\t\t<\/span><\/div>","aioseo_breadcrumb_json":[{"label":"Home","link":"https:\/\/www.lifetein.com\/blog"},{"label":"Peptide","link":"https:\/\/www.lifetein.com\/blog\/category\/peptide_synthesis\/"},{"label":"Revolutionary LIPSTIC Method with LPETG Peptide Illuminates Receptor-Ligand Interactions In Vivo And In Vitro","link":"https:\/\/www.lifetein.com\/blog\/lpetg-peptide-and-srta-reaction-allows-analysis-of-cell-cell-interactions-both-in-vitro-and-in-vivo\/"}],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/1247","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=1247"}],"version-history":[{"count":0,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/posts\/1247\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media\/1526"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=1247"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/categories?post=1247"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/tags?post=1247"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}