{"id":2740,"date":"2026-02-27T22:13:04","date_gmt":"2026-02-28T03:13:04","guid":{"rendered":"https:\/\/lifetein.com\/blog\/docs\/how-to-prepare-the-cy3-and-cy5-labeled-peptides\/"},"modified":"2026-02-27T22:51:01","modified_gmt":"2026-02-28T03:51:01","password":"","slug":"how-to-prepare-the-cy3-and-cy5-labeled-peptides","status":"publish","type":"docs","link":"https:\/\/www.lifetein.com\/blog\/docs\/how-to-prepare-the-cy3-and-cy5-labeled-peptides\/","title":{"rendered":"How to prepare the Cy3 and Cy5 labeled peptides?"},"content":{"rendered":"<ol>\n<li>Peptides were synthesized using standard Fmoc solid-phase peptide synthesis.<\/li>\n<li>After coupling, Lys(Mtt) was selectively deprotected to expose the side-chain amine moiety. Cy3 or Cy5 were dissolved in DMF, activated, and coupled to the free amine. Cy3\u2014and Cy5-labelled peptides were cleaved and HPLC purified according to the standard procedure.<\/li>\n<li>Lyophilization yielded a red powder for Cy3 and blue for Cy5.<\/li>\n<li>Stock solutions of non-labeled peptide (10 mM), Cy3-labelled peptide (1 mM), and Cy5-labelled peptide (1 mM) were prepared in water, aliquoted, and stored at \u221220 \u00b0C.<\/li>\n<li>Excitation wavelengths of 520 or 615 nm for Cy3 or Cy5, respectively, were used.<\/li>\n<li>Use emission wavelengths of 600 or 710 nm for Cy3 or Cy5, respectively.<\/li>\n<\/ol>\n<p><!-- liveagent_urlcode:238904 liveagent_entry_id: --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Peptides were synthesized using standard Fmoc solid-phase peptide synthesis. After coupling, Lys(Mtt) was selectively deprotected to expose the side-chain amine moiety. Cy3 or Cy5 were dissolved in DMF, activated, and coupled to the free amine. Cy3\u2014and Cy5-labelled peptides were cleaved &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/docs\/how-to-prepare-the-cy3-and-cy5-labeled-peptides\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_crdt_document":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"doc_category":[328],"doc_tag":[],"class_list":["post-2740","docs","type-docs","status-publish","hentry","doc_category-peptide-synthesis"],"aioseo_notices":[],"year_month":"2026-04","word_count":109,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"lifetein","author_nicename":"new_blog","author_url":"https:\/\/www.lifetein.com\/blog\/author\/new_blog\/"},"doc_category_info":[{"term_name":"Peptide Synthesis","term_url":"https:\/\/www.lifetein.com\/blog\/docs-category\/peptide-synthesis\/"}],"doc_tag_info":[],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs\/2740","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=2740"}],"version-history":[{"count":1,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs\/2740\/revisions"}],"predecessor-version":[{"id":2777,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs\/2740\/revisions\/2777"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=2740"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/doc_category?post=2740"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/doc_tag?post=2740"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}