{"id":2737,"date":"2026-02-27T22:12:58","date_gmt":"2026-02-28T03:12:58","guid":{"rendered":"https:\/\/lifetein.com\/blog\/docs\/elisa-standard-curve\/"},"modified":"2026-02-27T22:50:56","modified_gmt":"2026-02-28T03:50:56","password":"","slug":"elisa-standard-curve","status":"publish","type":"docs","link":"https:\/\/www.lifetein.com\/blog\/docs\/elisa-standard-curve\/","title":{"rendered":"ELISA standard curve"},"content":{"rendered":"<h1><strong>ELISA standard curve<\/strong><\/h1>\n<p><strong>Standards and the standard curve<\/strong><br \/>\n1. Make up a stock solution of 0.08 ug of protein\/100 ul of PBS and store at 4 C<\/p>\n<p>To make the stock:<\/p>\n<ul>\n<li>Use protein extracted from fresh hyphae that are nearly 100% immunoreactive. To determine this, run a Bradford and an ELISA assay on the samples and compare concentrations.<\/li>\n<li>If Bradford and ELISA values are nearly the same, make an ELISA curve and test the values by comparing results to a known curve.<\/li>\n<li>Make up 500 ul aliquots of the stock with a concentration of 0.08 ug of protein in 100 ul of PBS or 0.40 ug of protein in 500 ul of PBS.<\/li>\n<\/ul>\n<p>2. Put 100 ul of the 0.08 ug protein\/100 ul of PBS in 2 of the wells and 50 ul PBS in the other ten wells.<br \/>\n3. Transfer 50 ul of the 0.08 ug sample to a neighboring well that has 50 ul PBS.<br \/>\n4. Mix 3-4 times with the micropipette by pulling the sample up and down.<br \/>\n5. Remove 50 ul from these two wells and transfer to 2 adjacent wells. Mix 3-4x. Repeat for these two wells.<br \/>\n6. After the third dilution, remove 50 ul from the two wells that have 100 ul and dispose of it.<\/p>\n<p><img decoding=\"async\" src=\"\/chat\/scripts\/file.php?view=Y&amp;file=ncrwu29xhd4hvofxmv03ajisyqgecwqj\" \/><\/p>\n<p><!-- liveagent_urlcode:543786 liveagent_entry_id: --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>ELISA standard curve Standards and the standard curve 1. Make up a stock solution of 0.08 ug of protein\/100 ul of PBS and store at 4 C To make the stock: Use protein extracted from fresh hyphae that are nearly &hellip; <a href=\"https:\/\/www.lifetein.com\/blog\/docs\/elisa-standard-curve\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_crdt_document":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"doc_category":[329],"doc_tag":[],"class_list":["post-2737","docs","type-docs","status-publish","hentry","doc_category-antibody-services"],"aioseo_notices":[],"year_month":"2026-04","word_count":188,"total_views":0,"reactions":{"happy":0,"normal":0,"sad":0},"author_info":{"name":"lifetein","author_nicename":"new_blog","author_url":"https:\/\/www.lifetein.com\/blog\/author\/new_blog\/"},"doc_category_info":[{"term_name":"Antibody Services","term_url":"https:\/\/www.lifetein.com\/blog\/docs-category\/antibody-services\/"}],"doc_tag_info":[],"_links":{"self":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs\/2737","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs"}],"about":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/types\/docs"}],"author":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/comments?post=2737"}],"version-history":[{"count":1,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs\/2737\/revisions"}],"predecessor-version":[{"id":2774,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/docs\/2737\/revisions\/2774"}],"wp:attachment":[{"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/media?parent=2737"}],"wp:term":[{"taxonomy":"doc_category","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/doc_category?post=2737"},{"taxonomy":"doc_tag","embeddable":true,"href":"https:\/\/www.lifetein.com\/blog\/wp-json\/wp\/v2\/doc_tag?post=2737"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}