cell viability | LifeTein Peptide Blog https://www.lifetein.com/blog LifeTein: Custom Peptide Synthesis Service Sun, 17 Nov 2024 03:01:44 +0000 en-US hourly 1 https://wordpress.org/?v=6.9.4 https://www.lifetein.com/blog/wp-content/uploads/2023/10/cropped-android-chrome-512x512-1-32x32.png cell viability | LifeTein Peptide Blog https://www.lifetein.com/blog 32 32 How to analyze the cell viability with peptides? https://www.lifetein.com/blog/how-to-analyze-the-cell-viability-with-peptides/?utm_source=rss&utm_medium=rss&utm_campaign=how-to-analyze-the-cell-viability-with-peptides Tue, 26 Jun 2018 21:55:56 +0000 https://www.lifetein.com/blog/?p=483 Cells were seeded on 96-well culture plates (10000 cells/well) and incubated in 100 μL of DMEM containing 10% FBS. The medium was then replaced with fresh medium containing 10% FBS, and a peptide solution was added to each well at … Continue reading →

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  • Cells were seeded on 96-well culture plates (10000 cells/well) and incubated in 100 μL of DMEM containing 10% FBS.
  • The medium was then replaced with fresh medium containing 10% FBS, and a peptide solution was added to each well at an appropriate concentration (for example 0.5uM, 1uM, 1.5uM, 2uM).
  • After a 2-h incubation, Cell counting kit-8 (CCK-8) was used according to the manufacturer’s protocol. Cell Counting Kit-8 allows sensitive colorimetric assays for determining cell viability in cell proliferation and cytotoxicity assays.
  • Cell viability was evaluated by the absorbance of formazan from each well, and 100% cell viability was calculated from the wells without peptides.
  • The results are the mean and standard deviation obtained from 5 samples.

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