| LT0423 |
| 411005100205 |
| Mouse IgG2a |
| GST.B6 |
| 50 μg |
| GST protein |
| Recognizes native and denatured forms of purified GST or GST fusion proteins |
| Protein A affinity chromatography from mouse ascites fluid |
| Dot, ELISA, IS, IP, WB |
| WB (with ECL): 10-50ng of purified GST or GST fusion proteins |
| WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
| Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
| Fig. 1:5000 (0.2µg/mL) Ab dilution probed against BL-21 bacteria lysate expressing the GST protein |