Anti-human Creatine kinase-MB monoclonal antibody (clone 8D10)

Catalog Number:
LT_R004
Quantity:
50ug
Immunogen:
Full length native human CKMB protein
IgG type:
Rabbit IgG
Clonality:
Monoclonal
Applications:
TIA, ELISA
Specificity:
Human CKMB
Formulation:
0.22 μM filtered solution of PBS, pH 7.4
Purity:
> 95% determined by SDS-PAGE
Storage:

This antibody is shipped at 4 °C. This product is stable for 12 months from date of receipt when stored at -20 °C to -70 °C. Avoid freeze/thaw cycles.

Preparation:

Monoclonal antibody is produced by immunizing rabbit with full length human CKMB and purified using protein A resin.

Hazard/Biohazard:

This antibody contains 0.09% sodium azide as preservative. Please handle and dispose the product properly. No known biohazard is associated with this product.

Background:

Creatine kinase (CK) is a dimeric enzyme expressed by
diverse tissues and cell types with calculated molecular
weight of 86 KD for dimer and 43 KD for monomer. CK
converts creatine to phosphocreatine by consuming ATP. It
has two distinct types of subunits CKM (muscle type) and
CKB (brain type), generating three combinations: CKMM,
CKMB, CKBB. CKBB is found in brain and smooth muscle
as well as in other tissues and cells such as neuronal cells,
retina, kidney, and bone. CKMM is predominantly expressed
in skeletal and cardiac muscles. CKMB heterodimer is found
prominently in heart and released upon myocardial infarction.
CKMB is an important serum marker for myocardial
infarction.

Images:


Fig. 1 ELISA

ELISA conditions
1) Antigen: human CKMB protein at 1.0 μg/ml
2) detection antibody: rabbit anti-CKMB monoclonal antibody (clone 8D10, LT_R004) at initial 1.0 μg/ml followed by 1:3 serial dilutions.

  • 4 Units in Stock

$250.00

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