Anti-human CCL18 Monoclonal Antibody (clone 12F3)

Catalog Number:
LT_R009
Lot Number:
 
Immunogen:
Full length recombinant human CCL18 protein
IgG type:
Rabbit IgG
Clonality:
Monoclonal
Affinity:
1.08 x 10-9 M
Applications:
TIA, ELISA, WB
Pairing antibody:
LT_R008, LT_R010
Specificity:
Human CCL18, minimum cross reactivity to other CC chemokines
Formulation:
0.22 μM filtered solution of PBS, pH 7.4
Purity:
> 95% determined by SDS-PAGE
Storage:

This antibody is shipped at 4 °C. This product is stable for 12 months from date of receipt when stored at -20 °C to -70 °C. Avoid freeze/thaw cycles.

Preparation:

Monoclonal antibody is produced by immunizing rabbit with full length recombinant human CCL18 and purified using protein A resin.

Hazard/Biohazard:

This antibody contains 0.09% sodium azide as preservative. Please handle and dispose the product properly. No known biohazard is associated with this product.

Background:

Chemokine (C-C motif) ligand 18 (CCL18) is a 69 amino
acid residue cytokine matured from an 89 amino acid
precursor protein found only in human. It belongs to the
CC chemokine family, with putative molecular weight of
7.8 KD. Its synonyms are PARC, DC-CK1, AMAC-1, and
MIP-4. CCL18 is produced majorly by antigen-presenting
dendritic cells, monocytes, and macrophages. Its production
is regulated by IFN-gamma, IL-10, IL-4, and IL-13.
Recently three receptors have been characterized for
CCL18: PITPNM3, GPR30, and CCR8. CCL18 primarily
regulates recruitment of the adaptive immune system and
attracts lymphocytes including T cells, B cells, and
dendritic cells. It is involved into fibrotic lung diseases,
allergy, autoimmune diseases, breast cancer, and acute
lymphocytic leukemia.

Images:


Fig. 1 Sandwich ELISA

ELISA conditions
1) capture antibody: rabbit anti-CCL18 monoclonal antibody (clone 6E12) at 2 μg/ml
2) detection antibody: rabbit anti-CCL18 monoclonal antibody (clone 12F3, LT_R009) at 0.5 μg/ml


Fig. 2 Western blot

Western blot conditions
Lanes: recombinant human CCL18 protein at 50 ng per lane. Antibody concentration: 1 μg/ml
Blocking and antibody dilution buffer is 5% skim milk (w/v), 1x TBS, 0.05% Tween-20.

Fig. 3 Affinity

Kon: 6.06 x 105/Ms
Koff: 6.99 x 10-4/s
KD: 1.08 x 10-9 M

 

  • 4 Units in Stock

$350.00

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